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Amplification of the circular DNA (RCA) was performed using the bacteriophage phi (ø) 29 DNA polymerase [Illustra™ Templiphi 100 Amplification kit (GE Healthcare Life Sciences)] according to the manufacturer's instructions. One to 200 ng of total DNA was dissolved in 5 μl of sample ...
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The authors would like to thank the Gauteng Department of Health, the National Department of Health, and the hospital's management for granting permission to conduct this study in the hospital. They also thanked all the registered nurses who participated in the study.References...
Extracted DNA from theLeptospiraspp. isolates and the kidney tissues were tested at the Yale University School of Public Health, USA, using the TaqMan Cador®LeptospiraqPCR commercial kit on the Rotor Gene®Q (Whitehead Scientific, Germany®) to hybridize with theLipL32gene region of pathogeni...
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