A polyclonal antibody directed against the zinc finger domain of BmGATA beta was also used in gel retardation assays to confirm that factor BCFI is indeed encoded by the BmGATA beta gene. Conceptual translation of a complete cDNA clone encoding the BmGATA beta protein revealed that this protein...
Crystals of PH-ATPPase (0.4 mm × 0.3 mm × 0.1 mm) were obtained in 3 weeks by mixing 1 μl of the protein solution and 1 μl of the reservoir solution containing 30% (vol/vol) polyethylene glycol 400, 100 mM Tris–HCl (pH 8.4), and 200 mM MgCl2. A drop was equilibrated ...
Domain analysis revealed that not only the GATA-type zinc finger region but also the downstream His 2 motif of AGP1 is required for binding activity, showing that the AGP has a novel GATA-type zinc finger domain. AGP1 can activate expression from promoters containing the AG-motif in tobacco...
Domain analysis revealed that not only the GATA‐type zinc finger region but also the downstream His2 motif of AGP1 is required for binding activity, showing that the AGP has a novel GATA‐type zinc finger domain. AGP1 can activate expression from promoters containing the AG‐motif in tobacco...