Immunology Characterization of the human inflammatory response to Gardnerella vaginalis MIDDLE TENNESSEE STATE UNIVERSITY Anthony L. Farone VickEricGardnerella vaginalis is a Gram-positive bacterium associated with bacterial vaginosis (BV), pelvic inflammatory disease, and preterm birth. BV is the most ...
Gardnerellavaginalisis difficult to characterize in terms of its microbiologic designation and its clinical relevance. By16S rRNAsequence analysis, it is sufficiently distinct to merit its own genus but is somewhat closely related toBifidobacteriumspecies, which are anaerobic gram-positive rods.281It is...
G vaginalis is therefore a relatively common isolate in patients with vaginal discharge. The concentration of aerobic and anaerobic bacteria ranged from 4.9-11.0 log10/g of secretion with an anaerobe-to-aerobe ratio of 10:1. Anaerobic bacteria, particularly anaerobic Gram-positive cocci (mean ...
The G. vaginalis Genome Contains an Orthologue of Known CDCs The CDC family is made up of more than 15 protein toxins produced by several distinct gram-positive genera (reviewed in (38)). The basic local alignment search tool (BLAST) was used to compare raw genomic DNA sequence data from ...
Detection of G.vaginalis specific gene fragment was used by polymerase chain reaction. Gram staining and bacterial culture were used to detect GV. A rapid diagnosis test of BV was used to detect the product of BV. RESULTS The positive rate of detection of clue cells, bacterial culture, PCR ...
Detection of Fructose-6-phosphate Phosphoketolase (F6PPK), a Key Enzyme of the Bifid-Shunt, inGardnerella vaginalis - ScienceDirect The detection of Fructose-6-phosphate phosphoketolase (F6PPK) in cellular extracts, is considered to be the most reliable indication that Gram-positive rod... F....
(1991)revised the vaginal Gram-stain criteria for BV first described by Spiegel by developing a numerical score based on semiquantization of Gram-positive rods, Gram-negative coccobacilli forms, and curved Gram-negative rods. Thesemorphotypeswere thought to representLactobacillusspp.,G. vaginalis, ...
Bacterial vaginosis is the most common gynecological disorder affecting women of reproductive age. Bacterial vaginosis is frequently associated with the development of a Gardnerella vaginalis biofilm. Recent data indicates that G. vaginalis biofilms are
The diagnosis of Gardnerella vaginalis infection on the basis of microscopic and cultural findings was compared. A total of 340 specimens of vaginal secretion were Gram stained and plated on a medium selective for Gardnerella vaginalis . Positive culture was obtained in 165 cases. Microscopy was ...
Of those women with intermediate flora on Gram stain during the course of the study 5/6 (83.3%) were positive for G vaginalis while 5/13 (38.5%) of those women with only normal Nugent scores were positive for G vaginalis. Thus, 61.5% of women with normal Nugent scores had no evidence...