The GAPDH reaction is reversible, hence, necessary for hepatic gluconeogenesis. The chapter discusses GAPDH as being a metabolic 'switching station', diverting carbon flow appropriately. There is discussion regarding the experimental analysis of GAPDH's enzymatic function, particularly in the use of ...
Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products: - TaqMan ...
This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of...
Name:Glyceraldehyde-3-phosphate Dehydrogenase,CAS:9001-50-7.Use:It is used to determine the biochemical research of ATP glycerol -3-phosphate coupling reaction. It is commonly used as a diagnostic enzyme in clinic to directly determine the activity of ph
Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars/ Validacao de genes de referencia para normalizacao de reacao em cadeia de polimerase quantitativa em estudos de estresse hidrico em cana-de-acucar ...
5×Reaction Buffer———100μl Reverse Transcriptase I (200 U/μl)———10μl 活性定义:以Poly(rA) Oligo(dT)为模板/引物,在37℃,10分钟内,掺入1 nmol的dTTP为酸不性溶物质所需要酶量定义为1个活性单位(U)。 质量控制 核酸外切酶残留检测:200 U本品和0.6 μg λ-Hind Ⅲ在37 oC下孵育1小时,...
3. Prepare a master PCR reaction mix as described below. Add enough reagents for two extra reactions to account for loss of volume. Add 18 μl of the master PCR reaction mix to each PCR reaction tube or well of the PCR plate.
The reaction mechanism has been intensively investigated.3–8 Although the role of GAPDH as a housekeeping enzyme is well studied, recent investigations revealed new properties of this enzyme. These include localization on the cell surface, binding to cellular molecules,9–14 and roles in apoptosis....
人GAPDH基因探针法荧光定量PCR试剂盒,PCR检测试剂盒是一种用于聚合酶链式反应(Polymerase Chain Reaction,PCR)的试剂盒,它包含进行PCR实验所需的各种试剂和成分。PCR是一种分子生物学技术,用于在体外条件下快速制造数百万至数十亿份特定DNA序列的拷贝。PCR检测试剂盒根据其用途和检测目标的不同,可以分为多种类型,如用...
5. Reaction Mix: Each Standard, Positive Control, Sample and Background Control well will require 50 µl of Reaction Mix. Prepare sufficient material for the total number of wells to be run, containing: / Reaction Mix Background Control Mix Assay Buffer 46 µl 48 µl Glyceraldehyde-3...