Yeast assays measure yeast cell growth enabled by the production of histidine from theFUS1-HIS3reporter, which is induced by the pheromone-response pathway following GPCR activation. Growth is quantified by addition of the fluorogenic reagent Fluorescein-Di-β-D-Glucopyranoside (FDGlu; Invitrogen/Ther...
All experimental data to support the findings of this study are available upon request by contacting the corresponding author. The authors have annotated the entire data-building process and empirical techniques presented in the paper. Acknowledgments The authors thanks for the cooperation to Luigi Vimer...