Sections were mounted on gelatin-coated slides and stained with thionin for determination of optic fiber and electrode placement. Some sections were left unstained and coverslipped to verify viral transfection on a confocal microscope (Fluoview FV1000, Olympus). Recording and data processing Unit ...
Next, the cell on the same slides was stained with DAPI (1 μg/ml) in DPBS (10 min). The image of the stained cells was recorded by a confocal microscope (FLUOVIEW FV3000, Olympus, Tokyo, Japan) and analyzed with ImageJ software using macros designed for automatic analysis. Statistical...
Additionally, cells grown on microscopic slides were stained with DAPI (4,6-diamidino-2-phenylindol) at different post-treat- ment periods, and the nuclei were inspected for the typical morphological appearance of chromatin conden- sation during late apoptosis [26]. TBB-treated cells were also ...
Slides were then washed with PBS for 5 min. After 10 minutes incubation, the peroxidase reaction was developed using 0.01% hydrogen peroxide in 0.05% diaminobenzidine tetrahydrochloride (DAB). Sections were counterstained with Mayer's hematoxylin and dehydrated in ascending grades of ethanol prior to...
The IF assay for type-specific Igti antibody to each ofthe GBS :rolypes utilizes acetone-fixed whole bacteria on slides and mono- ~ecificrabbit antihuman IgG conjugated with fluorescein isothi- Lyanate ( 21 ). mg of G G or 0.03 to 4.8 mg of GBSlG in 0. I ml. 0. l to 0.5 ml ...
. Dehydration was performed using ascending concentrations of ethanol, ending in Xylenes. Slides were mounted with coverslips using Permount Mounting Medium (Thermo Fisher Scientific, Waltham, MA, USA, SP15-500) and imaged under light microscopy using a Keyence imaging instrument....
Static water-contact angle measurements were performed on samples coated on glass slides (from a 0.8% aqueous solution) by means of a Laurell (WS-650-23NPP) spin coater (Laurell Technologies Corporation, North Wales, PA, USA) under ambient conditions. The contact-angle experiments were run ...
After permeabilization and blocking, the slides were incubated with anti-γ-H2AX (Cell Signalling Technology Danvers, MA, USA) and Alexa594-conjugated secondary antibody with 4′,6-diamidino-2-phenylindole (DAPI) staining. For immunoblot analysis, cells were lysed with Laemmli’s buffer and then ...
Cryostat sections (10–12 μm) were cut at −20 °C, mounted on glass slides, air-dried for 2–3 h, fixed for 10–15 min in 1% paraformaldehyde (pH 7.4), air-dried again for 30 min, and stained with antibodies to TCR γδ, Ly6C, Ly6G, or CD8 (clones as above). A positive...