serve as a proxy for tOheurtorteasluilntshisbuigtigoenststtrheantgtthhethPaEtThbasasbeedenGsAuBgAgeAstreedcetpotoarlsodeanffseitcyt the gamma efficacy and ftrheeqvuaernicoyu3s1.nIettwiso, rhkokwienveemr,atuincsclperaorpheortwiesthtehaGt AhaBvAeAbereencesphtoorwndetnosiitnyfluliennkcsettoheGfAre...
To test this hypothesis, we generated an M1 mutant (M1Δ SP) by removing the 18-aa signal peptide from the protein (Fig. 4A). M1 without the peptide lost its ER-specific distribution (Fig. 4C). Importantly, expres- sion of M1Δ SP did not activate luciferase expression from ...
(***p < 0.001, One-way ANOVA with Tukey’s post-test). Full size image Next, we tested whether muscle-specific ERRγ activation protected against insulin resistance and glucose intolerance by performing insulin (ITT) and glucose (GTT) tolerance tests. We found a significantly impaired ...
To test whether the decrease in body weight gain in mice treated with metformin16 was due to changes in energy expenditure, we conducted indirect calorimetry analyses on HFD-fed mice treated with two doses of metformin (25, 50 mg/kg/day) to examine whole body energy expenditure. Real-...
eIFNγ production, compared to unstimulated cells. Although the differences between ConA and PWM did not reach statistical significance, a slight increase in eIFNγ was observed in the ConA-treated group. Therefore, ConA was chosen as a positive control in the IGRA for the real sample test. ...
Insulin and glucose tolerance test. Tests were performed as described. 3.5 U/kg of insulin (Sigma-Aldrich) was used for ITT57. For GTT, blood was collected in EDTA-coated tubes. Glucose and insulin was measured in plasma using colorimetry (Cayman Chemical) and ultrasensitive ELISA (...
Keywords: high-pulse radiation; switching-detection circuit; radiation test evaluation; radiation tolerance; total ionizing dose effects; electronic equipment 1. Introduction Gamma rays that are instantaneously incident in the form of high-energy pulses in environments exposed to radiation, such as outer...
(Thermos Fisher Scientific, Waltham, MA, USA). The following primers were used: (1)AnxA1, forward: 5′-TGTATCCTCGGATGTTGCTGCC-3′, reverse: 5′-CCATTCTCCTGTAAGTACGCGG-3′; (2)TSG6, forward: 5′-CTTGGCTGACTATGTAGA-3′, reverse: 5′-TTCCTGTGCTAATGATGT-3′; (3)PPARg, forward...
(p) G ) (x() ,), (2) where p data((•∙) and p G((∙•) )hhaavveevvaalulueessbbeetwtweeenn00aanndd11,,mmeeaannininggtthhaatttthhee cclloosseerr tthhee ddiiss-- criminnaattiioonn rreessuulltt ffoorr G is to 0, the closer the value of D opt((•∙)) is to...
International Journal of Molecular Sciences Article Prenatal Dexamethasone and Postnatal High-Fat Diet Decrease Interferon Gamma Production through an Age-Dependent Histone Modification in Male Sprague-Dawley Rats Hong-Ren Yu, You-Lin Tain, Jiunn-Ming Sheen, Mao-Meng Tiao, Chih-Cheng Chen, Ho-Chang ...