Preparation of polyclonal anti-ADAM17 peptide antibodies. Three days after the final injection, the mice were sacrificed, and blood and spleens were collected. Sera were separated by centrifugation, and IgG
For example, subjects suffering from an autoimmune disease such as Crohn's disease, lupus or psoriasis, are identified using any of a variety of clinical and/or laboratory tests such as, physical examination, radiologic examination and blood, urine and stool analysis to evaluate immune status. For...
Berchtold et al., Therapy of chronic idiopathic thrombocytopenic purpura in adults. Blood, vol. 74, No. 7, pp. 2309-2317 (Nov. 15, 1989). Brighton et al., Prospective evaluation of the clinical usefulness of an antigen-specific assay (MAIPA) in the idiopathic thrombocytopenic purpura and ot...
Three days after the final injection, the mice were sacrificed, and blood and spleens were collected. Sera were separated by centrifugation, and IgG was purified using Ab-Rapid spin column (ProteNova, Tokushima, Japan). All ADAM17 peptides were conjugated with NHS-Activated Agarose (Thermo Scie...
2.6. Cytokine Assays At the time of sacrifice, blood samples (0.8–1 mL) were obtained from MHV-68-infected mice, the blood was placed into chilled tubes containing ethylenediaminetetraacetic acid (EDTA), centrifuged, decanted, and the isolated plasma was then stored at −80 ◦C. To ...
Both Prenatal Dexamethasone Treatment and Postnatal HF Diet Changed the Leukocyte Subsets of the Rats at the Adolescent Stage There were no significant differences between the VEH, DEX, VHF, and DHF groups in terms of total leukocyte count, red blood cell count, and platelet count at D120. In...
The test speed was set at 1 mm/min. Using tungsten contents from 50 wt.% to 630.1w. Mt.%ec,hathneicatel nPsriolpeerstireesn gths showed a slight increase. After the complete melting of PEEK to form colloids, small amounts of tungsten aggregated in the colloid with supporting ...