On the 6th day, PBMC cultures were boosted for 4 h by addition of 1 µg/mL ionomycin and 50 ng/mL phorbol-12-myristate-13-acetate; 10 µg/mL brefeldin A was added to prevent secretion of IFN-γ. Thereafter, PBMC of duplicate cultures per condition were pooled and 180 µL of ...
Protein analysis by WESTERN blotting and ELISA. Tissues were homogenized in Pierce IP Lysis buffer (Thermo Scientific) using a polytron instrument at 25,000 rpm. Further the lysates were pre-cleared at 16,000 g, 20 min at 4 °C; and the supernatants were store at −80...
Muscle ERRγ over-expression successfully triggered glycolytic-to-oxidative myofiber switch, increased functional mitochondrial content and boosted vascular supply in the db/db mice. Despite aerobic remodeling, ERRγ surprisingly failed to improve whole-body energy expenditure, block muscle accumulation of ...
All nanoemulsion formulations underwent centrifugation at 3500 rpm and 25 °C for 30 min to detect any phase separation. Only those formulations that exhibited no phase separation were subjected to further examination. To assess physical stability, a heating−cooling cycle was implemented by storing ...
Although the possibility of performing non-destructive and fast measurements has been investigated in the past decades [10–13], recent developments in cyber-physical systems boosted by investments in Industry 4.0 sectors and supported by the cloud technology are opening new perspectives in the field....
The biological activity of γ-Fe2O3 magnetic nanoparticles (MNPs), obtained by the laser target evaporation technique, was studied, with a focus on their possible use in biosensor applications. The biological effect of the MNPs was investigated in vitro