作者以之前理性设计构建得到的色氨酸生产菌株S028为出发菌,构建PTS缺陷和GalP / Glk依赖型大肠杆菌菌株。但是,S028突变体的生长严重受损。为了解决这个问题,进行了针对GalP / Glk的基因表达调控的启动子筛选,辅以分批式适应性实验室进化(ALE...
Title:CombinatorialmodulationofgalPandglkgeneexpressionforimprovedalternativeglucose utilization AppliedMicrobilogyandBiotechnology JiaoLu 1,2 ,JinleiTang 1,2 ,YiLiu 1,2 ,XinnaZhu 2 ,TongcunZhang 1 ,XueliZhang 2* 1 TianjinUniversityofScience&Technology 2 KeyLaboratoryofSystemsMicrobialBiotechnology,Institute...
Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization Jiao Lu, Jinlei Tang, Yi Liu, Xinna Zhu, Tongcun Zhang and Xueli Zhang Phosphoenolpyruvate (PEP) is an important precursor for anaerobic production of succinate and malate. Although inactivating PEP/...
Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization. Appl Microbiol Biotechnol, 2012, 93: 2455-2462.Lu J,Tang JL,Liu Y. Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization[J].Applied Microbiology and ...
Zhang, Combinatorial modulation of galP and glk gene expression for improved alternative glucose utilization. Appl Microbiol Biotechnol, 2012. 93(6): p. 2455-62. 10.1007/s00253-011-3752-yJiao Lu,Jinlei Tang,Yi Liu,Xinna Zhu,Tongcun Zhang,Xueli Zhang. Combinatorial modulation of galP ...
Current generation from glucose or lactate by MR-1 (pBBR-glk-galP).Gen, NakagawaAtsushi, KouzumaAtsumi, HiroseTakuya, KasaiGen, YoshidaKazuya, Watanabe