6. 在同基因小鼠模型中,抑制Gal3协同CD47阻断抑制肿瘤进展 研究者通过将带有标记GFP荧光素酶的KP-Luc2小鼠胃腺癌细胞种植到小鼠体内,然后分别使用TD139、αCD47和联合处理来观察肿瘤生长情况。结果发现联合治疗显著减少了肿瘤生长,增加了吞噬作用和F4/80巨噬细胞的浸润。此外,联合治疗还显著增加了CD8+ T细胞和细胞...
研究者通过将带有标记GFP荧光素酶的KP-Luc2小鼠胃腺癌细胞种植到小鼠体内,然后分别使用TD139、αCD47和联合处理来观察肿瘤生长情况。结果发现联合治疗显著减少了肿瘤生长,增加了吞噬作用和F4/80巨噬细胞的浸润。此外,联合治疗还显著增加了CD8+ T细胞和细胞毒性T细胞的浸润和产生的IFNγ,同时增强了M1型巨噬细胞标志物...
This process starts with galectin-3 recruitment into intraluminal vesicles (ILVs), which are later released at the plasma membrane as exosomes. Electron microscopy is utilized to determine the location of GFP-tagged galectin-3 in pelleted exosomes. We also describe how these vesicles are harvested...
By expressing only the Carbohydrate Recognition Domain region of Gal3 fused with Photoactivatable GFP, we will be able to utilize Gal3 as a marker of ruptured vesicles in vivo.J. LondonDissertations & Theses - Gradworks
(Fig.1d). Moreover, immunofluorescent staining in HEK-293T cells showed that GFP-fused galectin-3 (GFP-Gal3) was co-localized with RagC, p18, and Lamp2 (lysosomal marker) (Fig.1e), supporting a notion that the interaction of galectin-3 with Rag GTPases and Ragulator occurs on ...
(further referred as WT GFP, n = 14), WT injected with AAV5-hSYN (further referred as WT hSYN, n = 14), Gal3KO injected with AAV5-GFP (further referred as Gal3KO GFP, n = 13) and Gal3KO injected with AAV5-hSYN (further referred as Gal3KO hSYN, n = 14)...
Huntington’s disease (HD) is a neurodegenerative disorder that manifests with movement dysfunction. The expression of mutant Huntingtin (mHTT) disrupts the functions of brain cells. Galectin-3 (Gal3) is a lectin that has not been extensively explored in
tin-3 clones was described previously [14]. All cells were cultured in Dulbecco’s modified Eagle’s medium with 10% heat- inactivated fetal bovine serum. 2.3. Transfection s The plasmid encoding full-length human Nup98 tagged with GFP was described previousl y [16,17]. The Nup98...
Samples of NTD or full-length galectin-3 at 500 µM in 500 mM or 1 M NaCl were mixed with 1% (5 µM) of NTD-GFP or with 1% GFP as control. NMR experiments and analysis NMR data were collected on Bruker AVIII 850-MHz or 600-MHz spectrometers, both equipped with a...
and tumors were treated ex vivo as described before for LB33-MEL xenografts. In SKBR3-Gal3GFP tumors, galectin-3 antagonists addition enhanced IFNγ-induced CXCL9/10 expression (Fig.3d; Supplementary Fig.3b). In contrast, SKBR3-Gal3mutGFP tumors did not express higher amounts of CXCL9/10...