Galactose-1-phosphate uridyl transferase (GALT), the deficient enzyme in classical galactosemia, was studied by Percoll-gradient age-fractionation of erythrocytes. For normal GALT, a rapid and substantial decrease in GALT activity and loss of most of two isozymes was found to occur in the ...
The middle enzyme of the Leloir pathway is galactose-1-phosphate uridyl transferase (GALT), which produces uridine diphosphogalactose (UDP-gal) from galactose 1-phosphate (gal-1P). UDP-gal can continue in the Leloir pathway to eventually be used for energy production, but it is also used to...
GALT is an important antigen of Actinobacillus pleuropneumoniae (APP), which was shown to provide partial protection against APP infection in a previous study in our lab. The main purpose of the present study is to investigate GALT induced cross-protection between different APP serotypes and eluci...
Classical galactosemia and mutations at the galactose-1-phosphate uridyl transferase (GALT) genedoi:10.1002/(SICI)1098-1004(1999)13:6<417::AID-HUMU1>3.0.CO;2-galactosemiamutationanalysisGALTClassical galactosemia is caused by a deficiency in activity of the enzyme galactose-1-phosphate uridyl...
It involves the sequential activities of (Fig. 1) galactokinase (GALK), galactose-1-phosphate uridyl transferase (GALT), and UDP-galactose 4-epimerase (GALE). Studies on galactose metabolism in Escherichia coli and Saccharomyces cerevisiae have mainly focused on the regulation of gene expression ...
Galactosemia (OMIM 230400), a life-threatening disorder with severe symptoms in the neonatal period, is caused by deficiency of the enzyme galactose 1-phosphate uridyl transferase (GALT [3]; EC 2.7.7.12). Accumulation of galactose 1-phosphate in the galactosemia fetus despite maternal milk avoida...
This chapter describes the determination of clinical aspects of galactose 1-phosphate uridyl transferase. Galactosemia, a hereditary disorder in humans, is characterized by a low level or a complete absence of the enzyme galactose 1-phosphate uridyl transferase (transferase) in blood cells, liver, an...
Mice deficient in galactose-1-phosphate uridyltransferase (GALT) demonstrate abnormal galactose metabolism but no obvious clinical phenotype. To further dissect the pathways of galactose metabolism in these animals, galactose oxidation and metabolite levels were studied in 16-day-old sucklings and the ...
Furthermore, -1,4-galactosyltransferase (GalT) from Neisseria meningitides was successfully overexpressed and purified by using an intein-mediated protein expression system. GalT was relatively stable at 25°C, and its activity was enhanced in the presence of DTT and BSA. Thus, it was feasible...
galtphosphateputscreenuridyltransferase8277582775Galactose-1-phosphate uridyltransferase is a blood test that measures the level of a substance called GALT, which helps break down milk sugars in your body. Low levels of this substance cause a condition called galactosemia.A.D.A.M. Editorial Board...