Zhu L: Yeast GAL4 two‑hybrid system. A genetic system to identify proteins that interact with a target protein. Methods Mol Biol 63: 173‑196, 1997.L. Zhu, Methods, yeast GAL4 two-hybrid system. A genetic system to identify proteins that interact with a target protein, Mol. ...
阐述酵母转录激活因子GAL4 的调控方式及其在酵母双杂合系统(yeast two hybrid system)中的应用 相关知识点: 试题来源: 解析 GAL4由两个结构域组成,一个是DNA结合结构域,一个是转录激活结构域。 可为将编码侍测蛋白 X的DNA序列同编码GAL4结合结构域的DNA序列克隆在一个载体上,表达产生X-GAL4杂合蛋白,将编码...
Options and considerations when using a yeast one-hybrid system[M]//Two-Hybrid Systems. Humana Press, New York, NY, 2018: 119-130. Stagljar I, Korostensky C, Johnsson N, et al. A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo[...
英文名:Y2HGold-GAL4 Yeast Two-Hybrid 储存条件:-80℃ RT 产品组成: 注: 1. 本试剂盒可供一次酵母筛库(质粒除外)。 2. 注意每个成份的保存温度,感受态细胞务必-80 ℃保存。 3. 0.5 L×3 代表:3 个包装,每个包装 0.5 L;5×1 mL 代表:1 个包装,含 5 个 1 mL。 4. 按照本试剂盒的流程操作...
酵母双杂交体系 Yeast two-hybrid system 酵母双杂交 (yeast two hybrid) 技术是利用酵母遗传学方法分析蛋白质之间的相互作用的技术. 背景知识 真核生物转录起始前区域结构: -25bp区有:TATA 序列 称为Hogness盒或TATA box,为启动子核心序列。 上游更远处 增强子 统称顺式作用元件 转录因子 能直接或间接辨认和结合...
GAL4Two-HybridSystem3LibrariesUserManualTableofContentsI.Introduction4II.Overview:AYeastTwo-HybridScreen8III.ListsofComponents9IV.AdditionalMaterialsRequired10V.YeastStrainsPhenotypes11A.YeastHostStrains11 B.Phenotypes11VI.ControlVectors13A.PositiveControls13 B.NegativeControl13VII.Matchmaker?GAL4cDNAGenomic...
matchmakerhybridhybridscreenclontechmatchmakertwoayeasttwo Matchmaker™GAL4Two-HybridSystem3&LibrariesUserManualPT3247-1(PR742219)Published3April2007Matchmaker™GAL4Two-HybridSystem3&LibrariesUserManualClontechLaboratories,Inc..clontechProtocolNo.PT3247-12VersionNo.PR742219 I. Introduction 4 II. Overview: A...
GAL4系统酵母双杂培养基套装+(含AbA,X-α-gal) 英文名: GAL4 Yeast Two-Hybrid Media plus Kit 储存温度: 序号 1-8 常温保存,序号 9 需-20℃储存,蓝冰运输; 序号 10 需 2-8℃储存。 产品组成:(0.5 L/pouch) 产品说
MatchmakerTwo-Hybrid System 3 is an advanced GAL4-based two-hybrid system that provides a transcriptional assay for detecting protein interactions in vivo in yeast.You can use this system to screen a library for novel proteins that interact with a known bait protein, or to test two previously ...
Yeast two-hybrid system 酵母双杂交 (yeast two hybrid) 技术是利 用酵母遗传学方法分析蛋白质之间的相互作 用的技术. 背景知识 真核生物转录起始前区域结构: -25bp区有:TATA 序列 称为 Hogness盒或TATA box,为启动子核 心序列。 上游更远处 增强子 统称顺式作用元件 转录因子 能直接或间接...