分子式: GAL4 DNA Binding Domain 产品名称: GAL4 DNA Binding Domain Polyclonal Antibody 产品规格: 100ul/200ul 有效成分含量: 100% 样本: 细胞、血清、血浆、组织匀浆等生物体液 保质期: 12个月 标记物: 无 储存条件: -20度保存 用途范围: 仅供科研使用 是否进口: 否 检测费用: 免费代...
After the assignment of 1H NMR signals of aromatic side chains by means of specific deuteration, we analyzed the DNA binding site of GAL4 by measuring photo-CIDNP spectra. The results showed that Trp36 is involved in both the specific interaction with UASG and non-specific DNA binding. This ...
酵母双杂交机制典型的真核生长转录因子,如GAL4、GCN4、等都含有二个不同的结构域: DNA结合结构域(DNA-binding domain)和转录激活结构域(transcription-activating domain)。前者可识别DNA上的特异序列,并使转录激活结构域定位于所调节的基因的上游,转录激活结构域可同转录复合体的其他成分作用,启动它所调节的基因的转...
首先，GAL4系统的核心是真核生物中常见的转录激活因子GAL4，其结构独特，分为DNA结合域（BD, DNA-binding domain）和转录激活域（AD, Transcriptional activation domain）。在双杂交实验中，我们将两个待测蛋白分别融合到BD和AD的载体上，如Bait（诱饵）和Prey（猎物）载体。当这两种融合蛋白在酵母细胞...
酵母双杂的原理基础:很多真核生物的位点特异转录激活因子通常具有两个可分割开的结构域,即DNA特异结合域(DNA-binding domain, BD)与转录激活域(Transcriptional activationdomain, AD)。这两个结构域各具功能,互不影响,但一个完整的激活特定基因表达的激活因子必须同时含有这两个结构域,否则无法完成激活功能。不同来源...
GAL4 DNA Binding Domain polyclonal antibody供应商 Bioworld产品货号 BZ16529产品报价 ￥询价/50ul产品说明书 点击查看购买方式 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868产品新闻背景资料 This protein is a positive regulator for the gene expression of the galactose-in...
Product name GAL4 DNA Binding Domain Polyclonal Antibody Immunogen Recombinant Protein Host Rabbit Reactivity Human Applications WB Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000-1:50000). Clonal...
酵母双杂的原理基础:很多真核生物的位点特异转录激活因子通常具有两个可分割开的结构域,即DNA 特异结合域(DNA-binding domain, BD)与转录激活域(Transcriptional activationdomain, AD)。这两个结构域各具功能,互不影响,但一个完整的激活特定基因表达的激活因子必须同时含有这两个结构域,否则无法完成激活功能。不同来...
DNA binding in experiments examining transcriptional activation functions of heterologous proteins This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site Fusions between GAL4 (aa 1-147) and activating ...
GAL4 DNA Binding Domain Polyclonal Antibody概述 产品信息 发表文章(0) 打印数据表 验证数据库 ¥1900.00元/format:100ul 目录号:IPB3142 库存:现货 规格 产品信息 Catalog No : IPB3142 Reactivity: - Applications: WB Dilution: WB: 1:10000-50000 Gene Name: - Protein Name: - Human Gene Id: -...