A DNA-segment containing a GAL 1 promoter linked to a gene outher than the gagactokinase gene for directing the expression of the gene within a yeast cell is new and may be used in a method of expression of a polypeptide in yeast, comprising introducing a GAL 1 promotor in a DNA-...
想做酵母诱导蛋白表达,有没有用过GAL1-10 Promoter的?这是个什么样的Promoter,序列可以从哪里找到呢...
Adoptive transfer of genetically engineered chimeric antigen receptor (CAR) T cells is becoming a promising treatment option for hematological malignancies. However, T cell immunotherapies have mostly failed in individuals with solid tumors. Here, with a
Promoters: Promoter for expression GAL1 Construction: pRS423, GAL1 promoter Marker(s):ampR,HIS3 Construct size (kb): 6.488999843597412 Features: marker(s): HIS3 marker(s): ampR promoter for expression: GAL1 replicon: 2 micron MCS: XhoI...SpeI ...
These patterns, characteristic of the induced wild-type GAL1 gene, were still galactose inducible with the TATA mutant GAl1 promoter, despite the low level of transcription from this promoter. We conclude that the GAL4-dependent protein/DNA structure responsible for the altered pattern within non...
The NPC-bound Mlp1 and Mlp2 proteins have been shown to associate with the GAL1 gene promoter and to maintain Ulp1, a key SUMO protease, at the NPC. Here, we show that the release of Ulp1 from the NPC increases the kinetics of GAL1 derepression, whereas artificial NPC anchoring of ...
目前最为常用的重组蛋白表达质粒载体融合了复制子(Replicon)、启动子(promoter)、筛选标签(selection marker)、多克隆位点(MCS)和融合蛋白移除策略(fusion tag removal strategy),如图1所示: β-1,3-半乳糖转移酶1(β3GALT1)重组蛋白问题3:成功表达一个重组蛋白,需要的策略组合: ...
A galactose-specific upstream activating sequence (UASG) that serves as a binding site for a galactose-specific transcription enhancing factor (the GAL4 gene product) is associated with the promoter for each of these genes. The binding domain is a 17 bp palindrome, which is repeated ...
The activity of p2 promoter is much higher than that of the p1 promoter in the colon adenocarcinoma cell line, COLO205. These results suggest that the hST3Gal II gene is expressed specifically by alternative promoter utilization and is regulated in a tissue-restricted fashion at the level of ...
We demonstrated that WDR5 increased H3K4 trimethylation (H3K4me3) modification at the promoter regions of GALNT10, facilitating recruitment of transcription factor ZBTB2 to the GALNT10 promoter. Collectively, our study uncovers an epigenetic upregulation of GALNT10 by BAG3 via WDR5 to facilitate ...