Deproteinization was performed by adding 5 μl of proteinase K at 10 mg.ml-1 and 2.5 μl of SDS 20% and incubated at 50°C for 1h. Samples were subsequently precipitated in ethanol, prior to two-dimensional separation on a 1.3% agarose gel as described in Clapier et al. (2001). ...
Genome sequencing and assembly Genomic DNA was extracted from a female laboratory-fed pupa using the sodium dodecyl sulfate (SDS) extraction method [30]. The SMRTbell library was constructed for sequencing using the SMRTbell Express Template Prep kit 2.0 (Pacific Biosciences). From this single indi...
Genome sequencing and assembly Genomic DNA was extracted from a female laboratory- fed pupa using the sodium dodecyl sulfate (SDS) extrac- tion method [30]. The SMRTbell library was constructed for sequencing using the SMRTbell Express Template Prep kit 2.0 (Pacific Biosciences). From this ...
end reads were generated. For the data analysis, raw data (raw reads) in fastq format were first processed. Clean data (clean reads) was obtained by removing reads containing adapters, reads containing N > 10% (N represents base that could not be determined) and low-quality reads from...