1c), supporting the reliability of our screen. Fig. 1: Human genome-wide gain-of-function screening identifies host factors regulating ZIKV infection in Huh7 cells. a Schematic of human genome-wide overexpression screen. b Scatter plot of screen candidates. The x-axis is log2 reads count ...
To identify host factors that promote flavivirus replication, we performed a genome-wide gain-of-function cDNA screen for human genes that enhance the replication of flavivirus reporter particles in human cells. The screen recovered seventeen potential host proteins that promote viral replication, ...
Genome-wide gain-of-function screen identifies novel regulators of pluripotency. Stem Cells 28: 1487- 1497; 2010.Abujarour, R., Efe, J., & Ding, S. (2010). Genome-wide gain-of- function screen identifies novel regulators of pluripotency. Stem Cells, 28(9), 1487-1497....
Here we perform a genome-wide gain-of-function screen using a weakly permissive NTCP-overexpressing Huh7-derived cell line termed Huh-106 cells5 and a genome-scale lentiviral open reading frame (ORF) library18, aiming to uncover HBV-related host-dependency fac- tors. We expect that the ...
Figure 1. Loss-of-Function Mutations in dACOX1 in Flies Cause Lethality (A) Schematic representation of the molecular lesion in dACOX1, the dACOX1 deletion (dACOX1ywg), and the insertional mutation (dACOX1T2A). (B) Summary of complementation tests. GR: Genomic Rescue construct. The numbers ...
We also tested the significance of embryonic expression of daughter gene Bmglv2, by RNAi which led to reduced hatching of embryos. This indicated that Bmglv2 has a role in embryonic development. We show that this gain of function in embryonic development was linked to loss of intronV. ...
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We report here that the potent HNF4α agonist N-trans-caffeoyltyramine (NCT) promotes weight loss by inducing an increase in mitochondrial mass and function, including fatty acid oxidation. Previously, we found in a short term trial in obese mice that NC
soluble proteins secreted by tumor cells25,26. In GBM, the intracellular function of HDACs is known to regulate the DNA damage response27and brain parenchyma invasion28by modulating the NF-κB and other pathways29, Nonetheless, the effects of HDACs on intratumoral spatial crosstalk remain unknown...
Keywords: mutant p53 (mutp53); missense p53; gain-of-function (GOF); p53 loss-of-heterozygosity (LOH); drug therapy; HSP90; HSF1 1. Introduction The tumor suppressor TP53 is the single most frequently mutated gene in over 50% of all human cancer patients. Alterations in p53 signaling ...