While the ABCG2 fold may be fully preserved in other ABCG and PDR family members, it will be worthwhile to test whether other members indeed share the same mechanism, since ABCG1, ABCG5/G8 and ABCG4 show a remarkably restricted sterol specificity. Noteworthy, the mechanism may be conserv...
To test this hypothesis, we performed a chromatin immunoprecipitation (ChIP)-quantitative PCR assay to analyze the enrichment of KDM4B and H3K9me3 at the CCNB1 promoter region. KDM4B was recruited to the proximal region of the CCNB1 promoter 4 h after IR treatment, the time point preceding...
To estimate the numbers of γH2AX foci in cells at G2, the nuclei of cells with bright cyclin B1 staining were chosen. To score γH2AX foci in cyclin B1-negative cells, the nuclei of cells with cyclin B1-negative cytoplasm were selected. To estimate the frequency of γH2AX foci, 100 -...
B2B marketers can pick and choose from different offline and online channels, and test the results to see which channels work best for them. A multichannel approach can be the best way to make use of limited marketing budgets – and create a system more powerful than the sum of its parts....