Guanine quadruplex (G-quadruplex) can bind tightly with hemin to form the hemin/G-quadruplex that displays robust peroxidase activity. Acting as a peroxidase-mimicking DNAzyme, hemin/G-quadruplex has been considered as a promising artificial enzyme for biosensing because of its low cost, high ...
这项成果以“The noncovalent dimerization of a G-quadruplex/hemin DNAzyme improves its biocatalytic properties”为题发表在Chemical Science上,论文的第一作者是博士生程昱和程明攀博士。 李灿课题组围绕 G4-DNA 酶催化功能的探索开展了系列研究工作,先后发现:人...
G4/hemin DNAzyme,当G4特异性结合hemin(Fe(III)-原卟啉IX)时,该复合物有趣地模拟了辣根过氧化物酶(HRP)的催化性质。作为HRP酶的代替物,该DNAzyme具有尺寸小、易合成和操作的优势,可通过变构调节来合理控制其活性,在生物分析和...
关键词:G・四链体;DNA酶;H92+;半胱氨酸;ATP;抗氧化性能 Abstract Abstract G。quadruplex—heminDNAzymes(G-quadruplexDNAzymes) are peroxidase‘likecomplexes formed by heminandsomenucleicacid G-quadruplexes. G-quadruplexDNAzymes can efficientlycatalyze ...
G_四链体_氯化血红素DNA酶在传感器设计中的应用_孔德明
四链体(G⁃quadruplex)。该G⁃四链体能够结合hemin(氯高铁血红素)形成具有过氧化物酶的活性 的G四链体⁃hemin复合物DNAzyme。将这一原理联合滚环扩增技术可以对核酸进行可视化的检 测。本研究旨在探索G⁃四链体⁃hemin复合物中,G⁃四链体结构以及两个G⁃四链体之间的链接长度 与DNAzyme过氧化物酶活...
Guanine quadruplex (G-quadruplex) can bind tightly with hemin to form the hemin/G-quadruplex that displays robust peroxidase activity. Acting as a peroxidase-mimicking DNAzyme, hemin/G-quadruplex has been considered as a promising artificial enzyme for biosensing because of its low cost, high therm...
Hemin/G-quadruplex-based DNAzyme concatamers as electrocatalysts and biolabels for amplified electrochemical immunosensing of IgG1. Juan Tang,Li Hou,Dianping Tang. Chemical Communications . 2012Tang J, Hou L, Tang D, Zhang B, Zhou J, Chen G. Hemin/G-quadruplex- based DNAzyme concatamers as...
G-四链体-heminDNA酶的应用研讨.pdf,中文摘要 中文摘要 G.四链体.氯化血红素(hemin)DNA酶(简称G.四链体DNA酶)是一种 由氯化血红素和特定的核酸G.四链体形成的具有过氧化物酶活性的人工模拟 酶。G.四链体.heminDNA酶可以有效的催化H202氧化ABTS(2,2’.氨基.二(3.
DNAzymeG-quadruplexphotooxidaseactive oxygen specieshypericinThe G﹒uadruplex (G4)/hemin entity has been widely used as a peroxidase﹎imic DNAzyme to catalyze the substrate oxidation with exogenous and concentrated H2O2 as oxidant for developing various devices. Herein, a G4‐based oxidase﹎imic ...