protein expression systemFc‐fusion proteinsmammalian expression systemsprokaryotic expression systemsviral expression systemsfinancial outlay by the owning party; however, these expenditures should be considered a valuable investment when protecting potentially very high value products. Beyond the basic science,...
2) Expression of fusion protein 融合蛋白表达 1. coli by transformation,the expression of fusion protein LacZ -280-α-ANP showed the highest level in host cell of JM101, when it grew at 37℃ for 14 h. 当带有此质粒的最适宿主菌JM101在37℃,270r/min摇床培养14h,融合蛋白表达量可达最高值。
protein molecule. Fusion protein expression is only one strategy for recombinant protein expression, and fusion expression is a method. Because fusion expression has the advantages of high expression efficiency, stable and water-soluble products, easy identification and purification, it has been widely ...
FURIN fusion protein Ag13261 SDS-PAGEEnlarge Image Country/Region Size Quantity Please contact our China branch Request bulk or custom quote Product Information Peptide Sequence YNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCP...
e.Fusion Proteins and Protein Cleavage Multifunctionalfusion proteinsare a good choice for co-expression provided that the domains of interest are active in similarsubcellular localizations(Fig. 11E). Some cytosolic proteins might also function when expressed as the cytoplasmic tail of a membrane-anchore...
This complete gene was expressed to a triple fusion recombinant protein using a bacterial expression vector under the control of robust bacteriophage T7 promoter. The recombinant DC Survivin-LTB protein, with a size of approximately 60kDa, was purified from the inclusion bodies using affinity based ...
These DNA fragments were ligated into the NdeI and EcoRI sites of pET21c and pET23a, respectively to construct the expression vectors pET21c-EYFP-TK_NBD-B and pET23a-EYFP-TB. Site-directed mutagenesis was performed by the overlap extension PCR method. The DNA sequences of these expression ...
Fusion protein expression and its insecticidal activity The truncated cry1Ab and the full-length vip3A gene were fused by a 24 base-pair nucleotide linker in reading frame to generate C1V3 gene. This fusion gene was inserted into pET28a vector and then transformed into E. coli BL21(DE3) str...
Steven Gygi of Harvard Medical School describes how the Orbitrap Fusion Lumos MS enables his lab to look at global protein expression levels to better understand biological processes and disease states. Quantitative ...
A series of versatile cloning vectors has been constructed that facilitate the expression of protein fusions to the Aequorea victoria green fluorescent pr... AGV Arnim,XW Deng,MG Stacey - 《Gene》 被引量: 523发表: 1998年 Enhanced expression of cro-beta-galactosidase fusion proteins under the con...