The puckering of the furanoid ring is C-3′- exo-C-4′- endo ( 3 T 4) [equivalent to C-2′- exo-C-3′- endo ( 2 T 3) in the numbering for d-ribose], with P and τ m angles of -6.5 and 42.7° respectively. Conformational analysis of the known ketofuranosides shows that...
PCR and DNA sequencing were used to verify the presence of the genes of interest in Pa5196 (data not shown); in the absence of a Pa5196 genome sequence, we use the PA7 gene numbering. Fig. 2 shows the phenotypes of the resulting Pa5196 single mutants with respect to twitching motility...