Protein methylation refers to the addition of a methyl group to the ε-amino group of proteins, with S-adenosyl-L-methionine (AdoMet) primarily used as the methyl group donor. Protein residues can be methylated on nitrogen (N-methylation), oxygen (O-methylation), sulfur (S-methylation), and...
Computational design of binding proteins does not have this limitation as a wide range of scaffolds can be used, with shapes more optimal to bind the target protein of interest. However, this advantage thus far has come with a different limitation—because of the inherent flexibility and lack ...
protein profilingCRISPR/Cas9endogenous affinity ligandsProtein tags have been essential for advancing our knowledge of the function of proteins, their localization, and the mapping of their interaction partners. Expressing epitope-tagged proteins has become a standard practice in every life science ...
The 5100 protein family: a biochemical and functional overview. In: Heizmann CW, ed. Novel Calcium-Binding Proteins. Fundamentals and Clinical Implications. Berlin: Springer-Verlag, 1991: 65–103. Chapter Google Scholar Zimmer DB. Examination of the calcium-modulated protein S 100a and its ...
Fig. 1. Genome organization of coronaviruses; Nsp3 domains and their functions. (A) The 5′-terminal two thirds of the CoV genome comprise ORF1a and ORF1b. ORF1a encodes the polyprotein 1a (Nsp1-11) while ORF1a plus ORF1b produce the polyprotein 1ab (Nsp1-16) through a ribosomal...
Protein phosphorylation participates in the regulation of all fundamental biological processes, and protein kinases have been intensively studied. However, while the focus was on catalytic activities, accumulating evidence suggests that non-catalytic pro
To date, twenty-one members of the PDI protein family have been identified, each having a different size, structure, tissue distribution and enzymatic activity (Table1) [11]. A signature of the PDI family members is the presence of a catalytic thioredoxin fold (TRX), also known as the CXX...
of protein models prediction of molecular interactions: from small ligands to large protein complexes macromolecular docking integrating prediction of structure, function, and interactions Prediction of Protein Structures, Functions and Interactions focuses on the methods that have performed well in CASPs, ...
The canonical model of RB-mediated tumour suppression developed over the past 30 years is based on the regulation of E2F transcription factors to restrict cell cycle progression. Several additional functions have been proposed for RB, on the basis of whi
The protein ERp57/GRP58 is a stress-responsive protein and a component of the protein disulfide isomerase family. Its functions in the endoplasmic reticulum are well known, concerning mainly the proper folding and quality control of glycoproteins, and pa