Methylation of nonhistone proteins affects protein stability, cellular localization, protein‒protein interactions, promoter binding affinity, and the regulation of other posttranslational modifications on the substrates (Fig. 1b). Fig. 1: Characteristics of protein lysine methylation. a The process of...
To evaluate the role of TAF15 in early neuronal development, we used human NPCs differentiated from iPSCs in which TAF15 protein levels and, for comparison, FUS protein levels, were individually depleted by lentiviral short hairpin RNAs (shRNAs;Fig. 5a,b). As TAF15 has a relatively minor ro...
Fig. 1. Genome organization of coronaviruses; Nsp3 domains and their functions. (A) The 5′-terminal two thirds of the CoV genome comprise ORF1a and ORF1b. ORF1a encodes the polyprotein 1a (Nsp1-11) while ORF1a plus ORF1b produce the polyprotein 1ab (Nsp1-16) through a ribosomal...
of protein models prediction of molecular interactions: from small ligands to large protein complexes macromolecular docking integrating prediction of structure, function, and interactions Prediction of Protein Structures, Functions and Interactions focuses on the methods that have performed well in CASPs, ...
The purified protein combines with the 4 S receptor-estrogen complex of the rat uterine cytosol to produce a 5 S receptor species. The E-RAF IgG cross-reacts with the nuclear 5 S receptor of the rat uterus but not with the cytoplasmic 4 S receptor. The E-RAF competes with the 5 S ...
To date, twenty-one members of the PDI protein family have been identified, each having a different size, structure, tissue distribution and enzymatic activity (Table1) [11]. A signature of the PDI family members is the presence of a catalytic thioredoxin fold (TRX), also known as the CXX...
The enzyme activity of each protein was determined by spectrophotometry and HPLC analyses. NpdI, a hydride transferase, catalyses a hydride transfer from reduced F420 to the aromatic ring of picric acid, generating the hydride σ-complex (hydride Meisenheimer complex) of picric acid (H-PA). ...
The protein ERp57/GRP58 is a stress-responsive protein and a component of the protein disulfide isomerase family. Its functions in the endoplasmic reticulum are well known, concerning mainly the proper folding and quality control of glycoproteins, and pa
The major isoform with the most abundant expression within a protein family. Acetylation A type of post-translational histone modification that facilitates or inhibits the binding of a protein complex to its histone-binding site via remodelling of the chromatin structure, resulting in altered gene expr...
The rapid evolution of viruses generates proteins that are essential for infectivity and replication but with unknown functions, due to extreme sequence divergence1. Here, using a database of 67,715 newly predicted protein structures from 4,463 eukaryoti