some extinct herbivores possess no close modern relatives and may have been functionally non-analog (e.g. 23 extinct ground sloths, 3 notoungulates, 4 diprotodons, 16
52 Cultures with >1 % ring-stage parasitaemia were centrifuged at 500 x g for 5 minutes at room temperature and the supernatant was discarded. Pellets were resuspended in 10 pellet volumes of pre-warmed (37°C) 5 % w/v sorbitol and incubated at 37°C for 15 minutes. The cell ...
For observation, a DM5500B digital microscope equipped with a 100× objective lens (N.A. 1.30) and DFC 310FX digital CCD color camera (Leica Microsystems GmbH, Wetzlar, Germany) was used. 2.5. Chromatin Immunoprecipitation (ChIP) Assay The ChIP assay was performed as described in [21]. ...