The intrinsic pK a -values of functional groups in enzymes: improper deductions from the pH-dependence of steady-state parameters. Crit. Rev. Biochem. 1976; 4 :165–173. [ PubMed ]Knowles, J.R. 1976 . The intrinsic pKa-values of functional groups in enzymes: Improper ded...
(1976). The intrinsic pKa-values of functional groups in enzymes: improper deductions from the pH-dependence of steady-state parameters. CRC Crit. Rev. ... Jeremy,R.,Knowles,... - 《Crc Critical Reviews in Biochemistry》 被引量: 64发表: 2008年 The Intrinsic PkA-Values of Functional Group...
However, values can be estimated by knowing the functional group in question, and if there is an additional inductive or resonance effects. When pKa is higher than pH? The pKa is higher than the pH if there is more of the basic form in a solution than the acidic form. This comes from...
We obtained quite different p K a values of adjacent serines in the tryptophan cage; the p K a value of the OH group of Ser13 exposed to bulk water is 14.69, whereas that of Ser14 not exposed to bulk water is 20.80 because of the internal hydrogen bonds. 展开 ...
H. Improved treatment of ligands and coupling effects in empirical calculation and rationalization of pKa values. J. Chem. Theory Comput. 7, 2284–2295 (2011). Article PubMed Google Scholar Jo, S., Kim, T., Iyer, V. G. & Im, W. CHARMM-GUI: a web-based graphical user interface ...
H. Very fast prediction and rationalization of pKa values for protein-ligand complexes. Proteins 73, 765–783 (2008). Article CAS PubMed Google Scholar Kohnke, B., Kutzner, C. & Grubmuller, H. A GPU-accelerated fast multipole method for GROMACS: performance and accuracy. J. Chem. Theory...
Ionizable lipids are an advanced delivery platform of gene therapy that can self-assemble intonanoparticleswhen mixed with polyanionic nucleic acids. Ionizable cationic lipids with modulated pKa values increase nucleic acid payload and enhance the therapeutic efficacy of gene therapy. At formulating step...
The Cys11 thiolate becomes protonated when theprotein unfolds, or the protein unfolds when Cys11 becomes protonated.The removal of the thiol group in the C14A had a large impact on theoutcome of the titrations while the K8A mutant only had a marginaleffect.Human spermatid-specific thioredoxin-...
P values (CTLA4 P = 1.1e-8, PD-1 P = 0.0015, Combo P = 3.2e-9). g, CT-26 PoleWT single cell clone1&2 tumors with ICB therapy (N = 15 mice per group). P values (Clone1 P = 0.023, Clone2 P = 0.053). h, Tumor inhibition rates of the CT-...
Electrostatic calculations of the pKa values of ionizable groups in bacteriorhodopsin. Journal of molecular biology 224, 473–486 (1992). 23. Bashford, D. & Karplus, M. pKa's of ionizable groups in proteins: atomic detail from a continuum electrostatic model. Biochemistry 29, 10219–10225 (...