The cuvettes were mounted in aluminum holders on top of an enclosed, computer-controlled sample chamber at 30 °C. For the variable temperatures (VTs) experiment (especially those involving ELK1 at 4, 30, and 45 °C), a thermostatted circulating water bath was fitted with the sample ...
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which cannot be physically separated from each other. This is possible only during the metaphase stage of cell division when the chromosomes are condensed. Early studies isolated single chromosomes from metaphase spreads of dividing cells using a micromanipulator (Chambers and...
ECM organization and mechanical properties. The traditional approach relies on experimentally exploring the effect of (a combination of) different input parameters (1,2). These experiments are very time consuming, labor intensive and therefore expensive. We propose a computer-aided approach which include...
[168] Zhou, J., Chen, S., & Jiang, S. (2003). Orientation of adsorbed antibodies on charged surfaces by computer simulation based on a united-residue model. Langmuir, 19, 3472–3478. DOI: 10.1021/la026871z. http://dx.doi.org/10.1021/la026871z10.1021/la026871zSearch in Google Scholar...
(Additional file4: Figure S1B), while ShK genes are composed of 2–3 exons. Additionally, large introns are present in all SA8 genes, but particularly so inA. tenebrosa, with a 17-kb intron identified in this species. Therefore, although ShK-like and SA8 peptides have a similar cysteine...
With this backdrop we examine here the TDCF by long computer simulations and mathematical modeling to understand functional co-ordination among residues. We show that the TDCF can explain the causal connection between the functional residues of Ub in the time scale of tens of nano-seconds. We ...
B, quantitative RT-PCR analysis of IL-8 (B) and MIP-3α (C) mRNA in TLR2 wild type and the DD loop mutant HEK 293 cells in response to8hof stimulation with 100 ng/ml of PAM3CSK4. Computer-assisted Docking of TIR Domains of TLR2 and TLR1—To gain structural insights into how ...
Alzheimer’s disease (AD) is the most common form of dementia and exhibits a considerable level of heritability. The bridging integrator 1 (BIN1) gene has recently been identified in several large genome-wide association studies (GWAS) as the second most
This task was performed on a computer in a quiet room outside the MRI scanner before performing magnetic resonance scanning for each subject, and the data were evaluated using E-Prime, Version 2.0 (http://www. pstnet.com/eprime.cfm) as described in our previous study (Liu et al, 2014)...