CONSTITUTION:The screen 5 is always magnetized during normal operation so that the foreign matter such as carbon or dust, etc., is stuck to the fine pores of the screen 5 and accumulated. When the accumulated amount is increased and the liquid passing area of the screen 5 is decreased so ...
Head kidneys were gently passed through a sterile cell strainer with 100 µm nylon mesh and rinsed with homogenization buffer (incomplete-DMEM medium containing 100 U/mL penicillin, 100 ug/mL streptomycin, 2% FBS, and 10 U/mL heparin). Cell suspensions were layered on 51% (1.07...
Afterwards, cells were dissociated using a pasteur pipette, and filtered through a 70 μm cell strainer, spun down and aspirated. Spleens were chopped and filtered. Small intestine was removed, cleaned from remaining fat tissue and washed in ice-cold PBS. Peyer’s patches were eliminated, ...
The tissue suspension was collected and passed through a 70-mm cell strainer and the cells were pelleted by centrifugation at 1,600 r.p.m. for 5 min. The nasal cells were then resuspended in RPMI. For analyses of NALT and non-NALT DCs separately, the NALT was dissected out as ...
Approximately 100–200 embryos were dechorionated with pronase (Roche), mechanically dissociated between 30 and 36 hpf through 100 and 70 mm cell strainers in 0.9 Â PBS solution supplemented with 10% fetal calf serum (FCS). Cell suspensions were analysed by fluorescence-activated cell sorting ...
Spleen and mesenteric lymph nodes (MLNs) single-cell suspensions were harvested by gently pushing cells through a 70-μm cell strainer with a syringe piston. First, cells were stained for extracellular markers and dead cells were excluded with fixable live/dead stain (Fixable Viability Dye Zombie...
containing 11% PURE Sucrose cushion buffer, 0.11% DTT and 0.02 % RNAse inhibitors) was added to each sample and after mixing, samples were filtered through a 30 µm Sysmex/ Partec CellTrics cell strainer (Wolf Laboratories, 04-004-2326). The filtrate was carefully pipetted on top of ...
Single-cell suspensions were prepared by gently sieving the spleen through a 70-μm cell strainer. Erythrocytes were removed using Pharm Lyse. Cell suspensions were washed twice with cold Hanks’ balanced salt solution to obtain leukocytes. Flow Cytometric Analysis Peripheral blood and spleen ...
Gut microbes are important regulators of host health and can also function as disease indicators. Lactiplantibacilllus plantarum(L. plantarum)used as express and delivery vaccines for mucosal immunity have been shown to activate specific immune responses
Next, Newport Green–stained cells were washed twice with 0.1% PBS and filtered using 5-ml tubes attached to a cell strainer cap. Cells positive and negative for Newport Green (excitation: 485 and emission: 530 nm) were sorted using a BD FACSAria Flow Cytometer (BD Biosciences). The ...