Root tips can sense moisture gradients and grow into environments with higher water potential. This process is called root hydrotropism. Here, we report three closely related receptor-like kinases (RLKs) that play critical roles in root hydrotropism: ALT
Briefly, samples were diluted 1:1 (v/v) in isopropanol, after which the supernatant containing organic solvents was evaporated at 90 °C for 30 min. The pellets were resuspended in 120 μL 95% (m/v) sulphuric acid (Merck) and incubated at 90 °C for 20 min. Next, the samples were...