Reverse transcriptase (RT) is a multifunctional enzyme that has RNA- and DNA-dependent DNA polymerase activity and ribonuclease H (RNase H) activity, and is responsible for the reverse transcription of retroviral single-stranded RNA into double-stranded DNA. The essential role that RT plays in ...
The ability of reverse transcriptases (RTs) to synthesize a complementary DNA from natural RNA and a range of unnatural xeno nucleic acid (XNA) template chemistries, underpins key methods in molecular and synthetic genetics. However, RTs have proven challenging to discover and engineer, in particula...
RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature 226, 1211–1213 (1970). CAS PubMed Google Scholar Schiff, R. D. & Grandgenett, D. P. Virus-coded origin of a 32,000-dalton protein from avian retrovirus cores: structural relatedness of p32 and the beta ...
The internal rotation potential energy curve around the glycosidic bond in 3′-azido-3′- deoxythymidine (AZT) molecule, an HIV-1 reverse transcriptase inhibitor, is calculated by using density functional B3LYP method and a 3–21G basis set. For the stationary points along the internal rotation ...
respiratory viruseshepatitis Bhepatitis Cinfluenzarespiratory syncytial virusMany important virulence genes of pathogenic bacteria are preferentially expressed in vivo... YR Kim,SE Lee,CM Kim,... - 《Infection & Immunity》 被引量: 515发表: 2003年 Biosynthesis of the reverse transcriptase of hepatitis...
HLA A2.1-restricted cytotoxic T cells recognizing a range of Epstein-Barr virus isolates through a defined epitope in latent membrane protein LMP2. J Virol 1993; 67: 7428–35. PubMed CAS Google Scholar Robbins PA, Lettice LA, Rota P et al. Comparison between two peptide epitopes presented...
Hepatitis B virus (HBV) is a para-retrovirus or retroid virus that contains a double-stranded DNA genome and replicates this DNA via reverse transcription of a RNA pregenome. Viral reverse transcription takes place within a capsid upon packaging of the RNA and the viral reverse transcriptase. A...
We reported previously that substitutions F61L, F61W, F61Y and F61A in human immunodeficiency virus type 1 (HIV-1) reverse transcriptase affect strand displacement synthesis [T. S. Fisher, T. Darden and V. R. Prasad (2003) J. Mol. Biol., 325, 443-459]. We have now determined the...
To validate RNA-seq results in terms of DEGs, RT-qPCR was performed by using a CFX96 Multicolor Real-time PCR Detection System (Bio-Rad). To this end, another RNA samples collected from the challenge experiment was reverse transcribed by using M-MLV reverse transcriptase (Promega, USA) and...
select article Structural basis for template switching by a group II intron–encoded non-LTR-retroelement reverse transcriptase Research articleOpen access Structural basis for template switching by a group II intron–encoded non-LTR-retroelement reverse transcriptase Alfred M. Lentzsch, Jennifer L. Stamo...