Fig. 4: Prediction of bound orientation of Proteinase K (PK) on AuNP. Full size image Subsequently, we tested our approach for predicting the orientation of unknown proteins. We chose two enzymes for this purpose, and we hypothesized that enzyme activity could be used to monitor protein orient...
Tail tissue was digested overnight at 55° C in the lysis buffer composed of 50 mM Tris-HCl (pH 8.0), 50 mM ethylenediaminetetraacetic acid (EDTA), 50 mM NaCl, 0.5% of N-Lauroyl sarcosine, 0.06% Tween 20, and 0.5 mg/mL of Proteinase K (Roche Life Science, Indianapolis...
Guanylate binding protein 1 (GBP1) is the most concerned member of the GBP family, which has a series of effects such as anti-infection and anti-angiogenesis. Its role in malignant tumors including cervical cancer is still controversial. We aim to explore the effects of GBP1 on cervical canc...
1995. Structure and possible function of aspartic proteinases in barley and other plants. In: K. Takahashi (Ed.) Advances in Experimental Medicine and Biology, Plenum, New York, pp. 241–254.Kervinen, J., Tormakangas, K., Runeberg-Roos, P., Guruprasad, K., Blundell, T. and Teeri, ...
After heat inactivation of proteinase K and centrifugation to remove cellular debris, DNA was precipitated by sodium acetate and ethanol. After centrifugation, DNA pellets were air-dried then resuspended in nuclease-free water. DNA concentration was analyzed using Nanodrop 2000 (ThermoFisher Scientific)...
(Sigma) in water. Mosquitoes and beads were homogenised using a disposable pestle and samples were incubated at 100°C for 10 minutes. Homogenates were spun at 20,000 xg for 1 minute. 1 μL of an 800 unit/mL stock of Proteinase K (Sigma) was added to 10 μL of the cleared ...
Preparation of nucleic acids Nucleic acids were isolated from 10,000–20,000 iL3s by a small-scale sodium proteinase K extraction [27] followed by purification using a mini-column (Wizard DNA Clean-Up System, Promega, USA). RNA was extracted from 20,000 ~ 30,000 iL3s using by TRizol ...
The TUNEL staining was conducted with the use of One Step TUNEL Apoptosis Assay Kit (Beyotime, C1088, China) according to the instruction. Briefly, the slices were incubated with proteinase K (20 μg/ml) for 30 min at 37℃ prior to staining with TUNEL detection liquid for 60 min at 37...
2019) removes free RNA from RNA that is bound to DNA through Hoogsteen pairing using a urea/NP40 extraction to isolate chromatin that is then treated with proteinase K to remove RNA bound to DNA via a protein intermediary. DNA:RNA triplex structures are further enriched using two complementary...
X-tremeGENE HP DNA Transfection Reagent (#XTGHP-RO), High Pure RNA Isolation Kit (#11828665001), and Proteinase K (#3115836001) were purchased from Roche (Switzerland). F-127 (#540025), Poly-L-lysine hydrobromide (#P6282), FITC-dextran (FD4, #60842-46-8), LPS (from Escherichia ...