Exploring the Function of Gene Promoter Regulatory Elements Using CRISPR ToolsGene promoters serve as pivotal regulators of transcription, orchestrating the initiation, rate, and specificity of gene expression, resulting in cellular diversity found among distinct cell types within multicellular organisms. ...
with promoter mutations to harnessing these regulatory components in biotechnological applications.In this chapter, we describe the manipulation of regulatory elements within promoters, with a specific focus on the use of CRISPR technology on enhancers and silencer elements of the Ovalbumin gene promoter....
The promoter region of NFL1 gene in tobacco was cloned by a novel PCR method,fused with the report gene GUS and transformed tobacco plants.The expression of GUS in the shoot apex of transgenic seedlings was detected.关键词: tobacco NFL gene promoter 被...
A previous study reported that both isoforms can function as repressors of reporter gene activity when bound proximal from a promoter13. CBX2.2 does not bind to CBX2.1 and was found to be significantly less active than the long isoform10,13. In humans, deficiency in CBX2 represents an ...
assay of the 3.1-kb promoter region revealed no significant difference in the luciferase activity present between the two parental lines B37 and P51 (Supplementary Fig.3). A conspicuous SNP from G/GT in B37 to C/GT in P51 was found in the 5′ splice site of the intron of the gene. ...
Cloning and Characterization of the 5′-Flanking Region of the Mouse Diastrophic Dysplasia Sulfate Transporter Gene could bind to this short segmentin vitro.BMP-2 increased the promoter activity as well as the nuclear protein binding to the sequence in C3H10T1/2 ... Tatsuya,Kobayashi,and,......
We suggest that σ 70 remains bound to core RNA polymerase when the 10 promoter contacts are broken, and then moves to the pause-inducing sequence. 展开 关键词: Escherichia coli Bacteriophage lambda DNA-Directed RNA Polymerases Sigma Factor DNA, Bacterial Transcription, Genetic Gene Expression ...
2002. The Pmed1 gene promoter of human Fc gamma RIIIA can function as a NK/T cell-specific restriction element, which involves binding of Sp1 transcription factor. J. Immunol. 168: 2857-2864... F Heusohn,G Wirries,RESE Gessner - 《Journal of Immunology Official Journal of the American ...
Mutual transcriptional regulation among these MYB genes makes it difficult to elucidate their individual function simply by analyzing knock-out mutants or ectopically overexpressing lines of these genes. In this study, we constructed transgenic lines expressing each MYB gene driven by its own promoter ...
In fact, within a typical cluster of IFNa-response genes, the interferon response factors are found to exclusively bind to the Epromoter, which is required for the proper induction of other genes within the cluster. Predictions based on gene expression dynamics and TF-binding profiles allow us ...