tear filmbreak-up timetear thinning testphenol red thread testThe study was designed in order to evaluate the tear film characteristics of elite athletes ... Llu??sa Quevedo,Gen??s Cardona,J Solé,... - 《International Contact Lens Clinic》 被引量: 5发表: 2000年 Evaluation of the phenol...
Repeatability of the phenol-red thread and tear thinning time tests for tear film function. Clin. Exp. Optom. 77, 64e68.Little SA, Bruce AS. Repeatability of phenol-red and tear thinning time tests for tear film function. Clin Exp Optom. 1994;77(2):64-68....
The depolarization of cardiac mitochondria was identified as a decreased red/green fluorescent ratio [23]. The level of mitochondrial swelling was determined from a suspension of isolated cardiac mitochondria using a microplate reader (Synergy HT, BioTek). Mitochondria (0.4 mg/mL) were incubated ...
Leaf weight was mea- sured at the indicated time point (n = 18). Curves were drawn based on the data from three independent experiments, and bars indicate standard errors. was withheld to cause a severe water deficit in soil. Af- ter 21 days, most of the control and mutant plants ...
Glycogen synthase kinase 3β (GSK3β) phosphorylates and thereby regulates a wide range of protein substrates involved in diverse cellular functions. Some GSK3β substrates, such as c-Myc and Snail, are nuclear transcription factors, suggesting the possibility that GSK3β function is controlled ...
Unrooted Phylogenetic Tree of 54 Proteins of the Cryptochrome/Photolyase Family The tree was constructed by the neighbor-joining (NJ) method. The cryptochrome DASH cluster is highlighted in red. The numbers at the nodes indicate the bootstrap probability for the clustering of sequences at the node...
(2, 4 and 6 h), a 30-μL sample was aliquoted and quenched by adding 1 M hydrochloric acid. For the serum stability assay, jS3 were incubated with 25% human AB-type male serum in phenol red-free Dulbecco’s modified Eagle’s medium at 37 °C for 24 h. At each time interval (...
Briefly, genomic DNA was digested with each of four restriction enzymes DraI, EcoRV, PvuII and StuI (BD Biosciences, Clontech). Each of the four digested products was purified by phenol/chloroform extraction and then linked to an adapter with T4 DNA ligase. Then, PCR was performed using ...
For full-length ER assays, MCF7 cells in phenol red-free DMEM with 10% charcoal-stripped FBS were transfected with 2.25 µg of HA-tagged, full-length ER expression vector, 6.75 µg of 3× ERE-TATA-luciferase plasmid (Addgene, catalogue no. 11354) and 0.67 µg of pR...
TH17 cells—a subset of CD4+T cells—exemplify immune adaptation. On the one hand, TH17 cells can have a full effector function protecting the host from pathogens with the associated risk of developing IMIDs1,2,3,4,5. On the other hand, TH17 cells can also acquire an anti-inflammatory fa...