Introduction Membrane proteins account for approximately 30% of the proteins encoded by a typical genome (Wallin and von Heijne 1998 ). This abundance gives some indication of the diversity of function of this class of protein. Typically, they are involved in regulating the cell's response to,...
We demonstrate that folded conformations of membrane protein complexes can exist in the gas phase. By resolving lipid-bound states, we then rank bound lipids on the basis of their ability to resist gas phase unfolding and thereby stabilize membrane protein structure. Lipids bind non-selectively and...
understanding of the molecular basis of cell membrane structure and function in normal and pathological states. Studies implicating protein-lipid interactions and (Na + K) ATPase activity in membrane alterations in disease states are also briefly discussed. Blazyk, J. F. and Steim, J. M., Bioch...
This lodges the protein in the interior of the bilayer. Proteins that are anchored by dissolving in the bilayer core are said to be integral to the membrane. At the same time, the tops and/or bottoms of these integral membrane proteins make contact with the water space. Predictably, these ...
The portions of membrane proteins that extend beyond the lipid bilayer into the extracellular environment are also hydrophilic and are frequently modified by the addition of sugar molecules. Other proteins are associated with the membrane but not inserted into it. They are sometimes anchored to lipids...
Protein trans -splicing (PTS) exerted by split inteins is a protein ligation reaction which enables overcoming the barriers of conventional heterologous protein production. We provide an overview of the current state-of-the-art in split intein engineerin
The pump protein retains its assymmetric orientation in the membrane. Formation of two-dimensional crystals suitable for image analysis can be induced by incubation in vanadate solution [6–8]. Immunologically the renal Na,K-ATPase is identical to the Na,K-pump in other mammalian tissues, ...
(Populustremula xtremuloidesSecretoryCarrier-AssociatedMembraneProtein3) gene. The SCAMPs are highly conserved 32–38 kDa proteins that are localized in the endomembranes and the plasmamembrane and that, in animals, seem to be mainly involved in exocytosis in specialized secretory cells but also ...
Chemical probes are selective small-molecule modulators, usually inhibitors, of their target protein’s function, that can be used in cell or even animal studies to interrogate the functions of their target proteins. Cheryl Arrowsmith, the leader of a ne
Epstein–Barr virus latent membrane protein 2 induces autophagy to promote abnormal acinus formation. J. Virol. 89, 6940–6944 (2015). Article CAS PubMed PubMed Central Google Scholar Inoue, J. et al. HBV secretion is regulated through the activation of endocytic and autophagic compartments ...