The formation and function of DNase I hypersensitive sites in the process of gene activationdoi:10.1016/S0021-9258(19)77625-XSarah C R Elgin
A New Function for an Old Enzyme: The Role of DNase I in Apoptosis Programmed cell death or apoptosis (Greek for the falling of the leafs in autumn) is an event by which cells are deliberately eliminated (recently reviewed... HG Mannherz,MC Peitsch,S Zanotti,... - Springer Berlin Heidel...
DNase I-hypersensitive sites I and II of the human growth hormone locus control region are a major developmental activator of somatotrope gene expression. ... IM Bennani-Baiti,SL Asa,D Song,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量:...
13, however its role in glandular apoptosis, B cell function, and sexual dimorphism has not been examined. In the present study, we sought to test the hypothesis that Th17 cells are unique antigen-specific T cells responsible for the development of autoantibodies, which target the destruction of...
Total RNA was extracted from cells using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The genes of interest were amplified from DNase I-treated, total RNAs using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA) and polydT primers. The ...
Transcription factors vs nucleosomes: regulation of the PHO5 promoter in yeast. Trends in Biochemical Sciences 22:93–7. CrossRef Thomas, G. H., and S. C. Elgin. 1988. Protein/DNA architecture of the DNase I hypersensitive region of the Drosophila hsp26 promoter. EMBO Journal 7:2191–...
Nucleosome cores have been crystallized and shown to be flat particles with dimensions of 110×110×57. Nucleosomes seem to persist in DNA replication and transcription. Judging from the fact that the DNA coding for active genes is preferentially digested by DNase I, the templateactive chromatin ...
11.1.1Electronmicroscopyofnucleicacidmolecules ElectronmicroscopyforstudyofDNARNAhydrids---todetermineifagenecontainsintrons.Fig11.3TheappearanceundertheelectronmicroscopeofaDNA-RNAhybridformedbetweenagenecontaininganintronanditsprocessedtranscript 6 Fig11.2PreparingaDNAmoleculeforobservationwiththeelectron...
Using stratified LD-score regression16, we found enrichment of all histone marks tested (H3K27ac, H3K9ac, H3K4me3 and H3K4me1) in lung- and smooth-muscle-containing cell lines (Supplementary Table 11). Using GARFIELD17 we assessed for enrichment of our signals for DNase l hypersensitivity ...
Total RNA was extracted from approximately 15 individuals per developmental stage using Trizol (Invitrogen, CA, USA) according to the manufacturer’s instructions. Then the total RNA was treated with RNase-free DNase I (Takara Bio, Japan) for 30 min at 37 °C to remove residual DNA. Nan...