The PcrA/UvrD helicase functions in multiple pathways that promote bacterial genome stability including the suppression of conflicts between replication and transcription and facilitating the repair of transcribed DNA. The reported ability of PcrA/UvrD to bind and backtrack RNA polymerase (1,2) might...
bHLHZipbasic region/helix-loop-helix/leucine zipper domain basic region/helix-loop-helix/leucine zipper domain PCRpolymerase chain reaction polymerase chain reaction EMSAelectrophoretic mobility shift assay electrophoretic mobility shift assay CDcircular dichroism circular dichroism NOEnuclear Overhauser effect nu...
select article High-salt transcription of DNA cotethered with T7 RNA polymerase to beads generates increased yields of highly pure RNA Research articleOpen access High-salt transcription of DNA cotethered with T7 RNA polymerase to beads generates increased yields of highly pure RNA Elvan Cavac, Lu...
Oxidation Resistance 1 (OXR1) gene is a highly conserved gene of the TLDc domain-containing family. OXR1 is involved in fundamental biological and cellular processes, including DNA damage response, antioxidant pathways, cell cycle, neuronal protection, a
The cDNA was amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs) with RNA PCR primer and RNA PCR index primers (Supplementary Data 1). The amplified products at the size of 130–160 bp were purified with 7.5% native PAGE, and then subjected to high-throughput ...
Thus, CRL4Cdt2 also regulates the subunit composition of Pol δ during the cell cycle. These studies reveal a novel function of CRL4Cdt2, i.e. the direct regulation of DNA polymerase δ, adding to its known functions in the regulation of the licensing of replication origins and expanding ...
C. An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell 101, 543–553 (2000). CAS PubMed Google Scholar Lipardi, C., Wei, Q. & Paterson, B. M. RNAi as random degradative PCR: siRNA ...
seem to largely depend on the degree of complementarity between seed sequences of miRNAs and the 3’ UTRs of target mRNAs. miRNA microRNA, Pol II RNA polymerase II, pri-miRNA primary transcript, DGCR8 DiGeorge syndrome critical region gene 8, pre-miRNA precursor miRNA, TRBP TAR RNA-binding...
B, mGluR mRNA expression as quantified by RT-PCR in Control iPSCs vs. neurons at day 49 of differentiation (n=2). C, Impact of 5 minutes of 50μΜ DHPG on calcium transients in human iPSC derived neurons. Graph represents number of neurons with active calcium transients compared to total...
An efficient protocol, termed background-minimized cassette (BMCM) by , has been developed for multiple of DNA. This method uses suitable extension primers for incorporating various mutation(s) and is not limited by either the nature of the mutation or the size and spatial location of mutationa...