To quantify the amount of PMA incorporated into PDCNP-Ab, the nanoparticles were dispersed in acetonitrile, followed by sonication for 1 h. After resting overnight, the PMA content was measured by HPLC using an LC-20AR HPLC system (Shimadzu, Japan) equipped with a UV detector SPD-20A, ...
The High-Performance Liquid Chromatography System (HPLC, Waters alliance e2695, Milford, USA) with UV detector (Waters 2468, Milford, USA) was performed using a Venusil ASB C18 (4.6 × 250 mm, 5 μm, China) set at a flow rate of 1 mL/min at 210 nm, 30 °C. The mobile ...
The effect of degree of roast on resulting caffeine content in brewed coffee has been extensively researched, but conflicting methodologies and results have muddled development of a general conclusion. In this study, 30 unique combinations of green coffee variety, degree of roast, and brew time were...
2,3. The bacterial chaperonin GroEL forms juxtaposed rings that bind unfolded protein and the lid-shaped cofactor GroES at their apertures. In vitro analyses of the chaperonin reaction have shown that substrate protein folds, unimpaired by aggregation, while transiently encapsulated in the GroEL centra...
139 Chromatographic methods (gas or liquid) serve as separation techniques, and the mass spectrometer serves as the detector; together, these methods achieve the highest analytical specificity and selectivity. In contrast to IAs, where one test measures a single steroid, a single run in an MS-...
The residues were dissolved in 0.05 to 0.10 ml of acetonitrile and stored at −20°C. The extracted hemes were analyzed by HPLC using a Waters 2795 separation module and Waters 2996 photodiode array (PDA) detector. For heme separation, a 1-mm-inner-diameter reverse-phase C18 column was ...
Analysis of nucleoside mixtures was performed on an API 4000 Q-TRAP mass spectrometer (Applied Biosystems) with an Agilent 1200 HPLC system and a diode array UV detector (190–400 nm) and equipped with an electrospray ionization source. The calibration curves for LC–MS/MS analysis are shown...
Methods: Subjects were selected by combination of disease diagnosis and Syndrome Differentiation. Plasma NE and E contents were determined by high performance liquid chromatography (HPLC) and electrochemical detector. The vegetative nervous function were assessed by comprehensive analysis of sympathetic and ...
The suspensions were then injected onto a Waters BEH amide column (2.1 ×150 mm, 1.7 µm particle size, 130 Å pore size) at 40 °C on a Dionex Ultimate 3000 UHPLC instrument with a fluorescence detector (λex = 310 nm, λem = 370 nm) coupled to a ...
The HPLC system was equipped with a C-18 column and the mobile phase was composed of a mixture of water and acetonitrile using a linear gradient elution. The UV detector was utilized at 210 nm. Methomyl was used as an internal standard. Water and synthetic medium were used as solvents. ...