如果您想填充应该观察到但没有观察到的缺失值,这非常有用。例如, full_seq(c(1, 2, 4, 6), 1) 将返回 1:6。用法 full_seq(x, period, tol = 1e-06) 参数 x 一个数值向量。 period 每次观察之间的差距。将检查现有数据以确保其确实具有此周期性。 tol 用于检查周期性的数值公差。
从建库测序原理上区分,主要有两大技术方案:一是以 drop-seq 为代表的3‘/ 5’端测序,这种方法虽然只能测到转录本一端大约600nt以内的序列,但是在大多数情况下,用部分序列代表整条分子用于 mRNA 的定量是可行的;第二种技术是以 smart-seq 技术为代表的...
Full-length mRNA-seq approaches capture the entire sequence of the mRNA transcript, providing more comprehensive transcriptome coverage for understanding gene expression patterns, alternative splicing events, gene regulation and function.SMART-Seq mRNA LP (with UMIs) The SMART-Seq mRNA LP (with UMIs)...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughpu...
The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells).This kit s...
Emerging methods for the accurate quantification of gene expression in individual cells hold promise for revealing the extent, function and origins of cell-to-cell variability. Different high-throughput methods for single-cell RNA-seq have been introduce
full-lengthhigh-throughputsingle cellThe single-cell RNA-sequencing (scRNA-seq) field has evolved tremendously since the first paper was published back in 2009 (Tang et al. Nat Methods 6:377-382, 2009). While the first methods analyzed just a handful of cells, the throughput and performance ...
Haplotype-resolved full-length transcriptome analysis in single cells by scCirRL-seq Updates (v0.0.1) What is scCirRL-seq Table of Contents Installation Operating system and python version Via conda (install locally for now) From source files 0. Preprocessing 0.1 Mapping 0.2 Transcript ...
Full-length RNA-seq from single cells using Smart-seq2. Emerging methods for the accurate quantification of gene expression in individual cells hold promise for revealing the extent, function and origins of cell... Simone,Picelli,R Omid,... - 《Nature Protocols》 被引量: 554发表: 2014年 ...
Here, we describe an automated protocol for full-length single-cell RNA sequencing, including both an in-house automated Smart-seq2 protocol and a commercial kit–based workflow. The protocols take 3–5 d to complete, depending on the number of plates processed in a batch. We discuss these ...