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However, fragments larger than 1000 bp (i.e., 2, 3, 4, and 5 kb) could not be inserted into the genome of CgInt. Therefore, for practical concerns, we had to use a plasmid-borne DNA template for gene insertion, and a linear template for gene deletion. In summary, we established ...
g PCR verification of the egfp insertion efficiency. Lanes 1–32 represent the PCR product of mutant (2711 bp), respectively. CK represents the PCR product of wild-type strain (1614 bp) Full size image Table 1 Summary of genome editing efficiency and plasmid curing efficiency of CRISPR-Cas9 ...
In 1943, Leo Kanner identified the core symptoms of Autism Spectrum Disorder (ASD) in young children [1]. Today, ASD is considered a highly heterogenous and lifelong neurodevelopmental disorder with onset in infancy or early childhood and diagnostic symptoms that include deficits in social communicati...
Reef manta rays (Mobula alfredi) are globally distributed in tropical and subtropical seas. Their life history traits (slow growth, late maturity, low reproductive output) make them vulnerable to perturbations and therefore require informed management st
The Gretag-Macbeth ColorChecker DC was launched in 2000 specifically for profiling Digital Cameras (DC). The number of colors had increased to 237 and also the color gamut covered by them was greatly expanded [28]. Eight of these patches were high-gloss, probably to cover a wider color gamut...
Table1showed the summary of the genetic diversity parameters for the different chicken breeds. It was observed that the Na values ranged from 2.23 for XY to 2.41 for CS, whereas, the Ne values ranged from 1.60 for SHXY to 1.73 for CS. And the range of PIC values in all chicken breeds ...
summary, the VIALE-A trial included 431 patients without history of exposure to azacitidine. At a median follow-up of 20.5 months, the median OS was 14.7 months in the azacitidine-venetoclax group and 9.6 months in the control group. The incidence of CR and composite complete remission rate ...
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In addition, tumors expressing decreased levels of the kinase reveal positive effects on overall survival when localized in the right colon compared to those in the left side. Conclusion In summary, this study provides evidence for the first time that CK1α RNA levels might serve as prognostic ...