150 μL of this cell suspension was transferred to a 96-well microtiter plate to perform sonication with an amplitude of 70%. After each 20 s of sonication, an interruption of 30 s followed. This sequence was repeated for six times and the 96-well microtiter plate was cooled permanently. ...
The commonly performed act of transferring a single animal to a new plate using a platinum wire pick inadvertently delivers an intense mechanical stimulus to the animal and evokes a characteristic behavioral response. It was previously shown that this mechanical stimulus leads to PVD- and FLP-mediate...