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However, at the molecular and metabolic level, the regulation mechanisms under P deficiency still remains incomplete. Combined omics analyses provides integrated and focused clues for intensive and comprehensive explanation of plant response mechanism. Transcriptome analysis under P deficiency stress will be ...
The leaves or roots were ground with a pre-cooled mortar and pestle in 2 mL ice-cold extraction buffer containing 50 mmol L−1 K2PO4-KOH (pH 7.5), 2 mmol L−1 EDTA, 1.5% (w/v) soluble casein, 2 mmol L−1 dithiothreitol(DTT) and 2% (w/v) insoluble polyvinylpolypyrrolidone ...
Analysis of other fruit development expression studies revealed that flavonoid pathway induction is conserved in the related Rosaceae species apple while lignin pathway induction is not. The transcription factor expression of peach genes homologous to known endocarp determinant genes in Arabidopsis including ...
of nuclease-free water was gently rinsed over the external surface of the stone. The stone was then transferred to a mortar and pestle that was sterilized with 5% sodium hypochlorite followed by RNase AWAY™ and pulverized into sand-like fragments. The fragments were suspended in 100 µL ...
APR, CS, ATPS, SiR, γGT, γGCS, Gly I and Gly II were extracted according to Lappartient and Touraine [61]. Briefly, six 6-mm-diameter frozen leaf discs or ~ 100 mg frozen root apices were ground with a precooled mortar and pestle in 1 mL ice-cold extraction buffer cont...
inneximosquitocidal toxin biosynthetic machinery, which we designated Xlt. In support of this hypothesis, two mutants each with an insertion in an Xlt biosynthesis gene cluster lacked the mosquitocidal compound based on HPLC/MS analysis and neither produced toxin to the levels of the wild type ...
SEM analysis of antagonistic B. amyloliquefaciens FLN13 interacting with hyphae of F. culmorum Fc1 on PDA medium at 5th day after incubation. a normal hyphae of F. culmorum Fc1; b, c damaged hyphae and macroconidia of F. culmorum Fc1; d damaged hyphae with cells of B. amyloliquefaciens ...
the dried peel was ground in a pestle and mortar. The milled powder sample was divided into two parts, one of which was used as powder (MP) and kept in a sealed vial at 4 °C. The other portion of the milled powder sample was used for extraction following a method described by Ho ...
old mice were snap-frozen in liquid nitrogen. for protein lysates generation, subsections of the liver and of the skin were grinded into a powder with a pestle and lysed for 1 h at 4 °c respectively with 400 and 200 μl ripa lysis buffer for 10 mg of tissue. 4x laemmli ...