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Before starting the genome assembly process, we conducted an extensive genome survey, which revealed an estimated genome size of 2.49 Gb with a heterozygous ratio of 0.30%. This genome size is comparable to otherOvisspecies. Following the assemble of PacBio reads and subsequent polishing with Illum...
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Metastatic colonization is one of the critical steps in tumor metastasis. A pre-metastatic niche is required for metastatic colonization and is determined by tumor-stroma interactions, yet the mechanistic underpinnings remain incompletely understood. PCR
Arrays were incubated with a PBS buffer based protein mixture of 10 nM His-tagged Myc/Max heterodimer or Max homodimer, 2% milk, 200 ng/?L BSA, 50 ng/?L Salmon Testes DNA, and 0.02% TX-100. Bound protein was tagged with 10 ng/?L anti-His antibody conjugated to Alexa 488 (Qiagen...
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betle smeared with oil are useful on the breasts of lactating women to promote milk secretion. In addition, the leaves can also be applied locally to cure inflammatory swelling such as orchitis, arthritis and mastitis; used to improve bad breath, body odor and prevent tooth decay; used to ...
RIPA lysate (Beyotime, Shanghai, China) was used to obtain total proteins from the mice aorta. Then a 30 µg protein sample was loaded in 12% SDS-PAGE gel and transferred onto 0.22 μm polyvinylidene difluoride membranes. The membrane was blocked with 5% milk at RT, and then incubated ...
[34] which found noM. bovisamong the 75 MTC isolates from the mainly cattle rearing communities of greater Mbarara. This could be a result of improved animal husbandry in Mbarara, or due to improved practices of the people such as boiling of milk before consumption. On the other hand, it...