Furthermore, NF investigates the relationship between the active ingredients of herbs in formulas and the biological molecular regulatory networks, delving deeper into the scientific meaning of the theory of herbal compatibility [2]. NP challenges the traditional paradigm of “one disease-one target-...
And the average false predictive value was 0.8, meaning 80% of predicted benign mutations were true negatives. In particular, MAGPIE identified pathogenic and benign variants successfully in the imbalanced datasets of stop-loss and stop-gain mutations. As shown in Fig. 3G, MAGPIE detected ...
Their structure and function are not completely elucidated. We have chosen zebrafish as a model to bring information about the role of collagen during embryogenesis. In the present study, we isolated four overlapping DNA complementary to RNA clones covering the 4879 nucleotides of a zebrafish ...
Cytidine deaminase is an enzyme that converts C-to-U through a hydrolytic deamination reaction (Fig.3). It is found in both prokaryotic and eukaryotic systems and has the ability to deaminate cytidine in both DNA and RNA. APOBECs, a class of enzymes that perform cytidine deamination, include...
The most stable structure of DNA is the canonical right-handed double helix termed B DNA. However, certain environments and sequence motifs favor alternative conformations, termed non-canonical secondary structures. The roles of DNA and RNA secondary str
TMZ exerts evolutionary selective pressure on GSCs, leading to the proliferation of drug-resistant GSCs characterized by enhanced DNA damage repair capabilities [3, 9]. Nevertheless, the mechanisms through which GSCs maintain self-renewal and derive chemoresistance are still not fully understood. ...
Telomeres are repetitive sequences (e.g., TTAGGG) within the DNA sequence at the end of chromosomes. Telomeres protect the coding regions of DNA from damage during normal cellular division and replication, and over time they become progressively shorter through telomere attrition [214,215]. The ...
of specific defense-related genes. Epigenetic mechanisms, including histone modifications and DNA methylation, which are closely linked to chromatin states, regulate gene expression associated with defense against biotic stress. Additionally, chromatin remodelers and non-coding RNA play a significant role ...
The gRNA contains a short region of RNA that is complementary to the target DNA sequence, which allows the gRNA to hybridize with the DNA and form a stable duplex [115]. This RNA–DNA hybridization is specific, meaning that the gRNA will only bind to a target DNA sequence that has a co...
SCLC patient tissue array, bioinformatics analysis were performed to demonstrate the role of periostin (POSTN) in SCLC progression, metastasis, and prognosis. Cell migration, invasion and sphere formation assay were performed to determine the oncogenic role of POSTN. RNA sequencing analysis was utilized...