coli were grown in LB overnight at 37 °C in a shaking incubator until cultures reached approximately OD600 = 5, as measured using a BioPhotometer Plus spectrophotometer (Eppendorf AG, Hamburg, Germany). Staining of hemocytes, pericardial cells, and heart muscle To fluorescently label ...
Ulceration of the oral mucosa also occurs, resulting in mouthrot. These mouth lesions are more lethal than are the skin lesions, since the painful oral lesions render the fish anorectic and lead to death due to starvation. Moreover, the disease spreads easily to the mandible and the maxilla...
The seeds have been germinated and grown in the greenhouse under standard conditions. The RNA has been isolated on the 16th of April 2013 from mature leaf samples from single genotype of each of the four species using the RNeasy plant mini kit from Qiagen according to the manufacturers ...
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farmed dulse is shown in Figure2. This particular specimen has an almost isotropic shape due to its being grown freely in a pool with constantly moving and swirling water. This is in contrast to dulse naturally grown by being anchored on a substrate at the bottom of the sea, which usually...
Cdk8 is a component of the mediator complex which facilitates transcription by RNA polymerase II and has been shown to play an important role in development of Dictyostelium discoideum. This eukaryote feeds as single cells but starvation triggers the for
For immunofluorescent staining, cells were grown on coverslips, after which they were fixed in 4% paraformaldehyde for 15 minutes at room temperature (RT). Coverslips were then washed briefly in PBST (90% phosphate-buffered saline (PBS), 10% fetal bovine serum (FBS), 0.05% Triton X-100),...
Despite rhabdoviral infections being one of the best known fish diseases, the gene expression changes induced at the surface tissues after the natural route of infection (infection-by-immersion) have not been described yet. This work describes the differential infected versus non-infected expression ...