Preparation of heat-killedStreptococcus pneumoniae(HKSP) and immunization S. pneumoniaestrain R36A, an avirulent, nonencapsulated strain, was grown to mid-log phase in Todd-Hewitt broth + 1% yeast extract (Becton Dickinson, Sparks, MD) and stored at − 80 °C. For immunization, sto...
there has been a rapid and accelerating growth of such Expressed Sequence Tags (EST). The initial study of 600 partial human sequences has grown to more than 20.0 × 106while more than 30 organisms have more than 100
the five cell lines (H2228, H838, H1975, HCC837, A549) were retrieved from the ATCC (https://www.atcc.org/) and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium with 10% fetal calf serum and 1% penicillin–streptomycin. The cells were grown independently ...
Paying for sex is widely accepted among men in Cambodia and the number of women engaged in sex work has grown substantially in the past few years; estimated at 34,193 [60]. Finally, our findings underscore the urgent need of for accessible HPV and cervical cancer prevention and treatment, ...
In most cases, BMP4 had no effect on the morphology of the cells grown in 3D environment, with the exception of MDA-MB-231 cells and MCF-10A cells. In PEG gel, MCF-10A cells formed irregular structures with small protrusions, the number of which increased upon BMP4 stimulation, indicatin...
Insect cells were grown and scaled up in commercial Sf-900 III SFM serum-free medium in a 27 °C incubator. A culture with 1.5 L of 2 × 106 Sf9 insect cells/mL was transfected with generated pGC-A virus with a MOI of 10. Seventy-two hours post-infection, the cells were harve...
HEK293FT cells (Invitrogen) were grown in DMEM medium (4.5 g L−1 of glucose and 6 mM L-glutamine, Gibco), supplemented with 10% FBS (Sigma-Aldrich), 0.1 mM MEM non-essential amino acids (Gibco), 1 mM sodium pyruvate (Gibco) and 100 µg ml−1 of pencillin–...
All cells were grown in RPMI with 10 % fetal bovine serum (FBS) at 37 °C with 5 % CO2. REN cells were grown in Ham’s F12 medium. All medium and FBS were obtained from Life Technologies or Sigma. Tissue samples Formalin-fixed paraffin embedded (FFPE) tumor tissues used in this ...
The SARS-CoV-2 virus, HCoV-19/Russia/StPetersburg-3524/2020 (Wuhan lineage, D614G, GISAID ID EPI_ISL_415710) was obtained from the Smorodintsev Research Institute of Influenza (Saint Petersburg, Russia). This virus was cultured in Vero (ATCC CCL-81) cells grown in DMEM supplemented with ...
HepG2, LNCaP, and MCF-7 cells were grown in 100 cm2dishes until they were approximately 80% confluent. After rinsing cells twice with cold PBS, two mls of protein extraction buffer (50 nM Tris Ph 8.0, 0.15 M NaCl, 0.5% NP40, 1× Halt Protease Inhibitor cocktail (#78430, Thermo Sci...