In our present study, we investigated the transcriptional response ofA. nigerstrain N402 grown on pretreated SB. The genome annotation of this strain was used for the microarray slides. However, this strain is more closely related to theA. nigergenomic DNA strain 3528.7 (ATCC 1015; American Type...
Cells (103 cells/well) were plated in triplicate samples in 24-well Ultra Low Attachment plates (Corning) in 1 mL of complete medium containing 0.75% methylcellulose (Sigma-Aldrich) and grown for 14 days before counting. In vivo tumorigenic assays The firefly luciferase gene was integrated into...
Akita T, Murohara T, Ikeda H, Sasaki K, Shimada T, Egami K, et al. Hypoxic preconditioning augments efficacy of human endothelial progenitor cells for therapeutic neovascularization. Lab Investig J Tech Methods Pathol. 2003;83(1):65–73. Article Google Scholar Hayakawa K, Chan SJ, Mandevill...
that it was not always possible to clearly distinguish between the two categories. In recent years, the way for the generation of designed microorganisms was paved by an increasing number of sequenced genes and even whole genomes, new bioinformatic tools providing the basis for analyzing this wealth...
The emergence of immune checkpoint inhibitors (ICIs), mainly including anti-programmed cell death protein 1/programmed cell death ligand 1 (PD-1/PD-L1) and anti-cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) monoclonal antibodies (mAbs), has shaped therapeutic landscape of some type of ca...
In that strategy, a tumor is resected and TIL are grown out of the tumor explant in vitro. Prior to T cell infusion, the patient is conditioned with a lymphodepleting regimen, and then is given IL-2 post infusion. Using this approach, response rates of 50% or greater have consistently...
Global climate models predict an increase in global mean temperature and a higher frequency of intense heat spikes during this century. Cereals such as rice (Oryza sativa L.) are more susceptible to heat stress, mainly during the gametogenesis and flower
With a capacity of 20 KW each, these new installations will bring the total micro-turbines to be delivered through this project to 20: six units in Hokkaido, 12 in Aomori and two in Akita. Their combined power will generate a total of 400 KW into the national grid: enough to supply ...
When Naoko (Kumi Takiuchi) proposes a one-night stand to Kenji (Tasuku Emoto) while he’sback in their hometown of Akita to attend her imminent wedding to a Self-Defense Forces officer, the fervid passion of their youthful romance over a decade earlier cannot be contained. Underemployed in ...
In temperate rice cultivation regions, japonica rice cultivars are grown preferentially because consumers deem them to have good eating quality, whereas indica rice cultivars have high grain yields and strong heat tolerance but are considered to have poo