An uncareful choice of the reference assembly version will impact the results of downstream phases; therefore, its selection must be considered in advance on the basis of the study purposes. For example, including alternative haplotypes of hypervariable regions, such as the major histocompatibility comp...
The technologies differ radically in how sequence data are generated (Fig.1). PacBio sequencing depends upon a DNA polymerase tethered to the bottom of a well of picolitre volume known as a zero-mode waveguide (ZMW) (Fig.1). Here, the DNA polymerase associates with a single molecule of n...
There was a need for innovation (E1: “It also forced us to get moving to hybridize…”) and teamwork in the form of collaboration with other departments and partners (E10: “It was very enriching because it led us, from a human and relational point of view, to work with our colleagues...
Amyotrophic lateral sclerosis (ALS) is caused by upper and lower motor neuron loss and has a fairly rapid disease progression, leading to fatality in an average of 2-5 years after symptom onset. Numerous genes have been implicated in this disease; however, many cases remain unexplained. Sever...
This cost was then inflated up to 2014, for a total of ZMW 103,467. With 30 CHWs trained, the cost per CHW is estimated at ZMW 3449 ($540 per CHW). Training for RCD activities is essentially an investment in human capital, so that the same process used to estimate an annual ...
NHS (National Health Service) 英国国家医疗服务体系 Here is Ms Truss’s first speech as PM in full: Good afternoon. I have just accepted her Majesty the Queen’s kind invitation to form a new government. Let mepay...
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JWW and ZMW reviewed and revised the manuscript and produced the final version. All authors read and approved the final manuscript. Corresponding author Correspondence to Jiawei Wang. Ethics declarations Ethics approval and consent to participate The study protocol adhered to the tenets of the ...
such as sequencing splices and low-quality data. Consistent sequence analysis is performed on each ZMW to obtain more accurate data, i.e., circular consensus sequencing data (CCS, also known as insertion reads), which will be used directly for assembly, alignment, species classification, etc. ...
Gray mold (Botrytis elliptica) causes a deleterious fungal disease that decreases the ornamental value and yield of lilies. Lilium oriental hybrid ‘Sorbonne’ is a variety that is resistant to gray mold. Understanding the mechanism of resistance against