MtDNAs are assembled with DNA-binding proteins such as TFAM to form mitochondrial nucleoid structures, and in live imaging, nucleoids dynamically move inside mitochondria and change their distribution and morphology. We previously reported that the dynamic features of nucleoids are regulated in cooperatio...
RNA was isolated form cells using the RNeasy Plus Mini Kit (Qiagen). For RNA sequencing, mRNA fraction was enriched using polyA capture from 200 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Next, mRNA libraries were prepared using the NEB Next Ultra II...
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Extracellular vesicles (EVs) have emerged as an exciting tool for targeted delivery of therapeutics for a wide range of diseases. As nano-scale membrane-bound particles derived from living cells, EVs possess inherent capabilities as carriers of biomolecu
Mesenchymal stromal cell derived extracellular vesicles (MSC-EVs) are a promising therapeutic for neuroinflammation. MSC-EVs can interact with microglia, the resident immune cells of the brain, to exert their immunomodulatory effects. In response to infl
PAHs are relatively insoluble in water, and they can bind to or form small particles in the air. This organic fraction also most probably caused the formation of aggregates in diesel exhaust samples visible in bright-field images. CNG-PM contains a higher amount of inorganic salts (NO3−, ...
UFP concentrations showed maximum concentrations ranging from 31,300 to 97,800 particles/cm3 when APU was selected on as a source of air on the ground and with engine bleed air and the air conditioning packs selected on during the climb. In 2 of the 4 flights the peaks were associated with...
TsiLang® Components Suite includes a number of highly professional, easy-to-use VCL components, wizards and tools for building multi-language applications under Delphi and C++Builder. On the one hand, our product dramatically simplifies the entire process of globalization, and on the other hand ...
EV are acting as a vehicle for cell–cell communication to modulate cellular activities and influencing the expression of their target genes in the receptor cells [28]. In recent years, MSC-derived EV are replacing MSCs themselves due to their paracrine functions, known as the new cell-free ...
The particle size distributions of TiO2 aerosols were measured using a cascade impactor (MOUDI, MSP Co., Shoreview, MN), an electrical mobility classifier (SMPS, TSI Inc., Shoreview, MN), and an aerodynamic sizing instrument (APS, TSI Inc.). The impactor was used for measuring mass-based...