Finally, 100 μL of TMB substrate and stop solution were incubated to visualize the immunoreaction, and the OD value was determined at 450 nm using a microplate reader. Immunofluorescence After dewaxing paraffin sections to water, the sections were repaired with EDTA buffer (pH = 8.0) and...
Lipid nanoparticles (LNPs) are crucial in nucleic acid drug delivery, enabling the transport of mRNAs and siRNAs into target cells [1]. They have gained prominence with the success of mRNA COVID-19 vaccines from Pfizer/BioNTech and Moderna, demonstrating their ability to elicit immune responses [...
constructing molecularly imprinted polymers that mimic protein like (or adapter like) binding bags and selective substrate bags [218], are employed to finely modulate the interaction of substrates with nanozymes,, which will be one of the promising methods to improve the high specificity of nanozymes...
Streptococcus suis (S. suis) is one of the most important porcine pathogens, causing severe pathologies such as meningitis or polyarthritis. It is also a very successful colonizer of mucosal surfaces. The IgM-degrading enzyme of S. suis (IdeSsuis) specif
Biotinylated 3D6 was incubated for 2 h at room temperature followed by 1 h of streptavidin-HRP (Mabtech, Nacka Strand, Sweden) at room temperature. ELISA was developed with K-blue Aqueous TMB substrate (Neogen Corp., Lexington, KY, USA) and read with a spectrophotometer at 450 nm. Samples...
Tests for Anti-HCV, though they may come in the rapid form of LFAs, cannot determine active infection. Thus, after Anti-HCV testing, which can take from 20 min to 3 days depending on the type of test used to return a result, an additional sample must then be taken for NAAT testing....
After washing with PBST, the bound antibodies were detected with tetramethylbenzidine (TMB) substrate (100 μl/well) at room temperature. The reaction was stopped with 2 M H2SO4 (100 μl/well). The optical density (OD) values were measured at 450 nm using a microplate reader (Thermo ...
Chemotherapy combined with or without targeted therapy is the fundamental treatment for metastatic colorectal cancer (mCRC). Due to the adverse effects of chemotherapeutic drugs and the biological characteristics of the tumor cells, it is difficult to ma
CIN, a hallmark of human cancer, results from errors in chromosome segregation during mitosis, leading to structural and numerical chromosomal abnormalities. This not only generates genomic heterogeneity that acts as a substrate for natural selection but also promotes inflammatory signaling by introducing ...
Since the discovery of Fe3O4 nanoparticles with enzyme-like activity in 2007, nanozymes have emerged as a promising class of catalysts, offering advantages such as high catalytic efficiency, low cost, mild reaction conditions, and excellent stability. Th