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CRISPR/Cas9-mediated targeted inactivation of p53 and Pten in mouse liver induced intrahepatic cholangiocarcinoma (ICC), a second form of primary liver cancer, in mice [19]. Multiplexing CRISPR/Cas9 has also been explored for high-throughput analysis of TSGs for initiation and development of liver...
encoding VSVg, two packaging plasmids encoding rev, gag and pol genes, and a transfer DNA plasmid vector, was added to pre-warmed OptiMEM (Thermo Fisher Scientific, 31985070), followed by addition of PEIpro and incubation for 30 min at room temperature for the transfection complexes to form....
We further examined the number of genes with at least one sequenced read in pseudo-bulk populations. For this purpose, cells form each cell type were pooled and gene-expression measurements were merged. We observed similar trends with higher numbers of detected genes with the 10x 3′ v3, ...
combinant form (URF), the circulating recombinant form CRF02_AG, sub-subtype F2, and subtype B, respectively. Out of 23 samples, 11 NFGS were obtained with a one-amplicon approach. Another 11 samples needeOdutwt oof a2m3 spalmicopnless,a1n1dNoFnGe Ssawmepreleoabttaleinasetdtwhriethe aa...
Vav1-Cre has been shown to direct Cre-mediated recombination in cells of the hematopoietic lineage [22]. Interestingly, our studies reveal that macrophages lacking TβRII have defects in expression of a set of genes that form the hallmark of the M2 polarizing program, suggesting that TGFβ ...
form of TGTGAN6TCACA. More recently, slightly different CRP binding sites with the consensus TGCGAN6TCGCA were also identified inE. coliand other γ-proteobacteria [22]. One of the major functions of CRP inE. coliinvolves the transcriptional regulation of genes related to organic carbon ...
50. Another possible explanation for the high frequency of the AG/TC repeat in the EST sequence data is that the AG/TC motif can appear in mRNA in the form of GAG, AGA, UCU and CUR codons, which are translated to the amino acids Arg, Glu, Ala and Leu, respectively; Ala and Leu ...