2g). Similarly, full-length coverage with Smart-seq3xpress resulted in significantly (2–5-fold) increased read support over exon–exon and exon–intron splice junctions, which form the basis for RNA velocity inference16 (Fig. 2h). To perform a direct comparison of cluster granularity, we ...
Cloning of NnWOX1-1, NnWOX4-3, and NnWOX5-1 The full sequences of the three genes were obtained from the NCBI database. The following primers were used: NnWOX1-1, forward primer: 5′-ATGGAAAGGAAAGATGAT-3′, reverse primer: 5′-TCACTTGGATTTGAATGG-3′. NnWOX4-3, forward pri...
Superoxide dismutases (SODs) are a key antioxidant enzyme family, which have been implicated in protecting plants against the toxic effects of reactive oxygen species. Despite current studies have shown that the gene family are involved in plant growth a
Microbiome alterations have been associated with depression, and fecal transfer of depressed patients’ microbiomes is sufficient to enhance despair behaviors in rodents. Yet little is known about the potential mechanisms, whereby microbes modulate depre
The DBD forms an N-terminal extension of the α-helices from each protein to grip the DNA in a manner comparable to forceps. This inserts into the DNA major groove where basic sidechains interact favourably with the DNA phosphate groups, while key residues form specific hydrogen bonding contacts...
Soft wheat contains high levels of both puroindoline proteins, puroindoline a (PINA) and puroindoline b (PINB), which form friabilin [3–5]. These small proteins are unique among plant proteins, having a cysteine-rich backbone and tryptophan-rich domain [6]. Their lipid-binding properties ...
It is divalent in all of its stable compounds and found abundantly in nature in the form of CdS. It may also combine with ammonia and cyanide to form Cd(NH3) −46 and Cd(CN) −24 , respectively. Various industrial processes such as mining, electroplating, stabilizing plastics, ...
(in absolute value) in the dKD vs. control comparison and a change in the opposite direction in the rescue vs. dKD comparison. For each category of events, we also distinguished two subclasses, depending on whether the 'inclusion' or 'exclusion' form of the event is enhanced when NMD is ...
2c) to the low PEP as in a monomeric form (Fig. 2d). Dimer mutation effect on RNA association and multimerization Our previous study on the CD1 domain alone suggested the dimer-interface residues FWKL (F126, W127, K180, L184) may be involved in both dimerization and RNA binding, ...
[6,7]. One extreme form of CNV is the presence-absence variation (PAV), in which a particular sequence is present in some individuals while absent in others. The advance of long- and short-read sequencing technologies has significantly boosted the identification of PAVs in numerous species [...