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glutamicum. Tailoring the central carbon and nitrogen metabolism enabled the efficient production of itaconic acid from acetate and therefore this study offers useful design principles to genetically engineer C. glutamicum for other products from acetate....
The underlying goal of synthetic biology is to make the process of engineering biological systems easier. Recent work has focused on defining and developing standard biological parts. The technical standard that has gained the most traction in the synthe
The machine and the machine can provide packaging form, compared with similar traditional packaging methods. In short, application of automatic wrapping machine, reduce labor intensity, reduce the labor resources, reduce the production process, its superior comprehensive per...
Malate is a C4-dicarboxylic acid widely used as an acidulant in the food and beverage industry. Rational engineering has been performed in the past for the development of microbial strains capable of efficient production of this metabolite. However, as m
2011). However, harsh industrial conditions certainly presume the exploitation of further enzyme sources. Moreover, conventional isolation and application techniques reached their limits in recent years, resulting in the development of versatile molecular biology techniques to engineer tailored biocatalysts (...
The natural mobility of the Ll.LtrB intron has been harnessed into a highly efficient gene targeting technology to achieve gene disruption through intron insertion in a number of gram positive and gram negative bacterial hosts [17, 18]. The ability to engineer a group II intron to insert at ...
but has to comprehensively include many other quality attribute requirements. Software quality attributes form the basis from which software engineers and architects develop software and make design decisions. Yet, there has been no quality attribute focused survey or classification of NoSQL databases wher...
We have therefore developed pHUSH, an inducible expression system that allows regulated expression of shRNA, miRNA or cDNA cassettes on a single viral vector. Results Both Pol II and Pol III promoters have been successfully combined with a second expression cassette containing a codon-optimized ...
(i.e., fragments thereof capable of promoter activity) in the form of a single MMV FLt DNA sequence or multiple enhancer domains of the MMV FLt DNA sequence and a coding sequence for a protein of interest; an expression vector containing the chimeric gene construct; a plant cell transformed...